Scholarlink

<p>The development of stable and efficient self-cascade catalytic systems for glucose detection remains a challenge in nanozyme-based sensing. Here, we report a self-cascade Au@CuMOF nanozyme, constructed by in-situ growth of gold nanoparticles (Au NPs) onto a copper-based metal–organic framework (CuMOF). The resulting Au@CuMOF possesses both glucose oxidase (GOx)-like activity derived from Au NPs and peroxidase (POD)-like activity derived from CuMOF. The negatively charged Au NPs electrostatically anchor onto the positively charged CuMOF surface, preventing aggregation and preserving their GOx-like activity. Leveraging these dual functionalities, Au@CuMOF enables a self-cascade reaction: the GOx-like activity catalyzes glucose oxidation to gluconic acid and H<sub>2</sub>O<sub>2</sub>, and the POD-like activity decomposes H<sub>2</sub>O<sub>2</sub> to generate hydroxyl radical (•OH) that oxidizes TMB to produce a blue colorimetric signal, eliminating the need for natural enzymes. The established colorimetric method exhibits a low detection limit of 2.52 µM for glucose, good selectivity, and satisfactory accuracy in spiked serum samples with recoveries ranging from 97.6% to 103.7%. This Au@CuMOF-based cascade sensor features superior stability over natural enzymes alongside facile operation and convenient integration, offering a feasible strategy to fabricate simple yet efficient enzyme-free self-cascading nanozyme sensors.</p> Graphical abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

A self-cascade nanozyme with glucose oxidase-/peroxidase-like activities via in situ growth of Au nanoparticles on CuMOF for colorimetric glucose detection

  • 期刊论文

  • 发表日期：

    2026年6月19日

查看全文
  • Minnan Zhu,
  • Guangyan Li,
  • Jie Cheng,
  • Fei Chen,
  • Yayang Tian,
  • Linfeng Chen

摘要

The development of stable and efficient self-cascade catalytic systems for glucose detection remains a challenge in nanozyme-based sensing. Here, we report a self-cascade Au@CuMOF nanozyme, constructed by in-situ growth of gold nanoparticles (Au NPs) onto a copper-based metal–organic framework (CuMOF). The resulting Au@CuMOF possesses both glucose oxidase (GOx)-like activity derived from Au NPs and peroxidase (POD)-like activity derived from CuMOF. The negatively charged Au NPs electrostatically anchor onto the positively charged CuMOF surface, preventing aggregation and preserving their GOx-like activity. Leveraging these dual functionalities, Au@CuMOF enables a self-cascade reaction: the GOx-like activity catalyzes glucose oxidation to gluconic acid and H2O2, and the POD-like activity decomposes H2O2 to generate hydroxyl radical (•OH) that oxidizes TMB to produce a blue colorimetric signal, eliminating the need for natural enzymes. The established colorimetric method exhibits a low detection limit of 2.52 µM for glucose, good selectivity, and satisfactory accuracy in spiked serum samples with recoveries ranging from 97.6% to 103.7%. This Au@CuMOF-based cascade sensor features superior stability over natural enzymes alongside facile operation and convenient integration, offering a feasible strategy to fabricate simple yet efficient enzyme-free self-cascading nanozyme sensors.

Graphical abstract