Scholarlink

<p>Accurate and species-specific diagnosis of <i>Plasmodium vivax</i> malaria remains a significant challenge in malaria management due to the insufficient sensitivity and selectivity of existing rapid antigen tests. In this study, a highly sensitive and selective lateral flow immunoassay (LFIA) platform was developed utilizing blue-colored cellulose nanobeads (CNBs) as high-contrast colorimetric reporters for the detection of <i>P. vivax</i> lactate dehydrogenase (PvLDH). Six novel monoclonal antibodies with sub-nanomolar dissociation constants and exceptional specificity toward PvLDH were generated and characterized. Systematic screening identified an optimal antibody pair—clone #6 for detection (probe) and clone #4 for capture—which was subsequently incorporated into the CNB-based LFIA. The developed assay achieved limit of detection values of 5 ng/mL in buffer and 10 ng/mL in blood sample, representing a 50- to 100-fold improvement in sensitivity compared with conventional gold nanoparticle-based commercial RDTs. Notably, the distinct blue signal produced by the CNBs provided superior visual contrast against the red background of the blood sample, enabling clear, instrument-free interpretation. The assay exhibited no cross-reactivity with <i>P. falciparum</i> LDH or human LDH, thereby ensuring robust species-specific discrimination. Given its high sensitivity, portability, and strong performance in complex biological matrices, this CNB-based LFIA represents a promising next-generation point-of-care diagnostic tool for effective <i>P. vivax</i> malaria detection in endemic settings.</p> Graphical abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Highly sensitive detection of species-specific malaria antigens using a cellulose nanobead-based lateral flow immunoassay

  • 期刊论文

  • 发表日期：

    2026年6月15日

查看全文
  • Yeon-Jun Kim,
  • So Yeon Kim,
  • Si-Eun Yu,
  • Sang-Oh Ha,
  • Jae-Won Choi

摘要

Accurate and species-specific diagnosis of Plasmodium vivax malaria remains a significant challenge in malaria management due to the insufficient sensitivity and selectivity of existing rapid antigen tests. In this study, a highly sensitive and selective lateral flow immunoassay (LFIA) platform was developed utilizing blue-colored cellulose nanobeads (CNBs) as high-contrast colorimetric reporters for the detection of P. vivax lactate dehydrogenase (PvLDH). Six novel monoclonal antibodies with sub-nanomolar dissociation constants and exceptional specificity toward PvLDH were generated and characterized. Systematic screening identified an optimal antibody pair—clone #6 for detection (probe) and clone #4 for capture—which was subsequently incorporated into the CNB-based LFIA. The developed assay achieved limit of detection values of 5 ng/mL in buffer and 10 ng/mL in blood sample, representing a 50- to 100-fold improvement in sensitivity compared with conventional gold nanoparticle-based commercial RDTs. Notably, the distinct blue signal produced by the CNBs provided superior visual contrast against the red background of the blood sample, enabling clear, instrument-free interpretation. The assay exhibited no cross-reactivity with P. falciparum LDH or human LDH, thereby ensuring robust species-specific discrimination. Given its high sensitivity, portability, and strong performance in complex biological matrices, this CNB-based LFIA represents a promising next-generation point-of-care diagnostic tool for effective P. vivax malaria detection in endemic settings.

Graphical abstract