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<p>The widespread contamination of cereal crops by zearalenone (ZEN) poses a threat to food safety, demanding sensitive and reliable analytical methods. In this study, a signal-on electrochemical aptasensor was developed based on a dual-amplification strategy combining a PEI-CoSe<sub>2</sub>/PtPd NPs nanocomposite with Exonuclease I (Exo I)-assisted target recycling. The PEI-CoSe<sub>2</sub>/PtPd NPs nanocomposite provided enhanced electrode conductivity and a large surface area for efficient immobilization of DNA probes. In the presence of ZEN, the aptamer dissociated from its complementary strand, triggering Exo I-mediated cleavage and enabling target recycling, which led to signal amplification. Under optimized conditions, the aptasensor exhibited a linear response over the range 0.001–100 ng/mL with a detection limit of 0.18 pg/mL. In addition, the proposed sensor also showed good selectivity, reproducibility, and applicability in spiked corn samples. These results indicate that the developed aptasensor provides a feasible approach for ZEN detection in food samples.</p> Graphical Abstract <p></p>

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Exonuclease I-driven target recycling strategy integrated with PEI-CoSe2/PtPd NPs for ultrasensitive electrochemical detection of zearalenone

  • 期刊论文

  • 发表日期：

    2026年5月9日

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  • Gengan Du,
  • Han Yan,
  • Weidi Kong,
  • Long Wang,
  • Baoshan He,
  • Qi Guo

摘要

The widespread contamination of cereal crops by zearalenone (ZEN) poses a threat to food safety, demanding sensitive and reliable analytical methods. In this study, a signal-on electrochemical aptasensor was developed based on a dual-amplification strategy combining a PEI-CoSe2/PtPd NPs nanocomposite with Exonuclease I (Exo I)-assisted target recycling. The PEI-CoSe2/PtPd NPs nanocomposite provided enhanced electrode conductivity and a large surface area for efficient immobilization of DNA probes. In the presence of ZEN, the aptamer dissociated from its complementary strand, triggering Exo I-mediated cleavage and enabling target recycling, which led to signal amplification. Under optimized conditions, the aptasensor exhibited a linear response over the range 0.001–100 ng/mL with a detection limit of 0.18 pg/mL. In addition, the proposed sensor also showed good selectivity, reproducibility, and applicability in spiked corn samples. These results indicate that the developed aptasensor provides a feasible approach for ZEN detection in food samples.

Graphical Abstract