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<p>Current detection methods for aflatoxin B1 (AFB1)&#xa0;are often hindered by interference from complex sample matrices and typically require bulky instrumentation and specialized operations, which limit their utility for rapid on-site screening. To overcome these challenges, a lateral flow assay (LFA) strip was developed that employs Prussian blue nanoparticles (PBNP) as a signal label. This approach substitutes conventional gold nanoparticles with cost-effective and easily synthesized PBNP. By utilizing EDC/NHS coupling, PBNP was conjugated to an AFB1-specific aptamer, establishing a competitive detection system. The experiments were optimized for probe coupling efficiency and reaction conditions, and the performance of the test strip was comprehensively evaluated. Results indicated that the constructed PBNP-LFA exhibited reliable detection performance, with a detection limit of 20.92&#xa0;µg/kg, and excellent specificity for AFB1, with the entire detection process completed within 10&#xa0;min. Furthermore, in practical food samples, such as corn flour and peanuts, this method demonstrated remarkable resistance to matrix interference and good reliability. This study presents a promising new strategy for rapid, low-cost point-of-care detection of AFB1.</p> Graphical abstract <p></p>

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Lateral flow assay for detecting aflatoxin B1 based on prussian blue nanoparticles

  • 期刊论文

  • 发表日期：

    2026年4月27日

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  • Ying Zhong,
  • Hao Zhang,
  • Yihan Yuan,
  • Bole Zou,
  • Chao Hu,
  • Qin Tu,
  • Jinyi Wang

摘要

Current detection methods for aflatoxin B1 (AFB1) are often hindered by interference from complex sample matrices and typically require bulky instrumentation and specialized operations, which limit their utility for rapid on-site screening. To overcome these challenges, a lateral flow assay (LFA) strip was developed that employs Prussian blue nanoparticles (PBNP) as a signal label. This approach substitutes conventional gold nanoparticles with cost-effective and easily synthesized PBNP. By utilizing EDC/NHS coupling, PBNP was conjugated to an AFB1-specific aptamer, establishing a competitive detection system. The experiments were optimized for probe coupling efficiency and reaction conditions, and the performance of the test strip was comprehensively evaluated. Results indicated that the constructed PBNP-LFA exhibited reliable detection performance, with a detection limit of 20.92 µg/kg, and excellent specificity for AFB1, with the entire detection process completed within 10 min. Furthermore, in practical food samples, such as corn flour and peanuts, this method demonstrated remarkable resistance to matrix interference and good reliability. This study presents a promising new strategy for rapid, low-cost point-of-care detection of AFB1.

Graphical abstract