<p>Three-dimensional (3D) printing has evolved from a prototyping tool into a versatile manufacturing platform for biochemical and environmental analysis. This review summarizes recent advances in the main additive manufacturing routes relevant to analytical applications, including fused filament fabrication (FFF), stereolithography (SLA), digital light processing (DLP), two-photon polymerization (TPP), and inkjet-based printing. We critically discuss how printing modality, material selection, and post-fabrication functionalization determine device performance in representative applications such as lab-on-a-chip systems, droplet microfluidics, enzyme-immobilized electrodes, DNA/RNA detection devices, wearable and portable biosensors, lateral flow platforms, and environmental monitoring systems. Particular emphasis is placed on the growing roles of hybrid manufacturing, nanomaterial integration, bioprinting, and AI-assisted sensing. Although substantial progress has been achieved, major translational barriers remain, including limited material libraries, insufficient biocompatibility and chemical stability, resolution and surface-quality constraints, lack of standardization, and incomplete regulatory pathways. Future development will depend on multifunctional printable materials, improved process control, integrated system design, and validation frameworks that support scalable, field-deployable analytical devices. Overall, 3D printing is emerging as a key enabling technology for next-generation analytical platforms across biomedical, food, and environmental applications.</p> Graphical Abstract <p></p>