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<p>Most of the detection methods for the triazole pesticide propiconazole hardly meet the requirements of its residue limits in different species. To solve this problem, t a one-step competitive colorimetric-fluorescence immunoassay for propiconazole detection assisted by gold nanoparticles (AuNPs) and red carbon dots (rCDs)&#xa0;was constructed. The proposed immunosensor demonstrated a wide linear detection range of 0.09–600 ng/mL (colorimetry) and 0.8–600 ng/mL (fluorescence), with detection limits as low as 0.01 ng/mL (colorimetry) and 0.8 ng/mL (fluorescence). The method was applicable to the detection of propiconazole residue limits in various foods (bananas, potatoes, lotus roots, corn) and the accuracy was verified by LC-MS. The versatility of this detection platform enables its expansion for the detection of different small-molecule hazards through the incorporation of target-specific antigens and antibodies.</p> Graphical abstract <p></p>

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One-step competitive colorimetric and fluorescence immunoassay for the determination of propiconazole pesticide assisted by gold nanoparticles and red carbon dots

  • 期刊论文

  • 发表日期：

    2026年4月21日

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  • Nan Wang,
  • Peng-Ru Li,
  • Ya Zhang,
  • Ya-Qi Yuan,
  • Guo-Yuan Xiong,
  • Zhen-Lin Xu,
  • Jing-Nan Meng

摘要

Most of the detection methods for the triazole pesticide propiconazole hardly meet the requirements of its residue limits in different species. To solve this problem, t a one-step competitive colorimetric-fluorescence immunoassay for propiconazole detection assisted by gold nanoparticles (AuNPs) and red carbon dots (rCDs) was constructed. The proposed immunosensor demonstrated a wide linear detection range of 0.09–600 ng/mL (colorimetry) and 0.8–600 ng/mL (fluorescence), with detection limits as low as 0.01 ng/mL (colorimetry) and 0.8 ng/mL (fluorescence). The method was applicable to the detection of propiconazole residue limits in various foods (bananas, potatoes, lotus roots, corn) and the accuracy was verified by LC-MS. The versatility of this detection platform enables its expansion for the detection of different small-molecule hazards through the incorporation of target-specific antigens and antibodies.

Graphical abstract