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<p>In gout progression, uric acid (UA) accumulation triggers crystal deposition, while interleukin-6 (IL-6) drives the subsequent inflammatory flares. Therefore, monitoring both UA and IL-6 is important for gout management. In this study, for the first time a microfluidic electrochemical system (MES) has been developed to simultaneously detect UA and IL-6 levels in blood. The MES consists of a multilayer microfluidic chip that incorporates a plasma separation module and two screen-printed electrochemical (SPE) sensors, integrated with a wireless potentiostat for real-time data analysis. The working electrode (WE) of one SPE was modified with uricase and hydrophilic fumed nano-silica (FSiO₂) for UA sensing, and the WE of the other SPE was modified with gold nanoparticle-reduced graphene oxide (Au NPs/rGO) composites and antibodies for IL-6 sensing. The MES demonstrated excellent analytical performance for the simultaneous detection of UA and IL-6 in human blood, featuring high selectivity, broad linear ranges (10-1000 µM for UA and 0.001-100 ng/mL for IL-6), robust stability, and strong concordance with reference methods (R² &gt; 0.95). This study provides a proof-of-concept platform for gout-related biomarker determination and offers a new strategy for the development of multi-biomarker electroanalytical devices.</p> Graphical Abstract <p></p>

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An integrated electrochemical microfluidic chip for simultaneous detection of uric acid and interleukin-6

  • 期刊论文

  • 发表日期：

    2026年4月9日

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  • Junbo Wang,
  • Joseph Benjamin Holman,
  • Xiangyu Chen,
  • Jiaying Sun,
  • Bensheng Qiu,
  • Anqing Zhang,
  • Weiping Ding,
  • Chengpan Li

摘要

In gout progression, uric acid (UA) accumulation triggers crystal deposition, while interleukin-6 (IL-6) drives the subsequent inflammatory flares. Therefore, monitoring both UA and IL-6 is important for gout management. In this study, for the first time a microfluidic electrochemical system (MES) has been developed to simultaneously detect UA and IL-6 levels in blood. The MES consists of a multilayer microfluidic chip that incorporates a plasma separation module and two screen-printed electrochemical (SPE) sensors, integrated with a wireless potentiostat for real-time data analysis. The working electrode (WE) of one SPE was modified with uricase and hydrophilic fumed nano-silica (FSiO₂) for UA sensing, and the WE of the other SPE was modified with gold nanoparticle-reduced graphene oxide (Au NPs/rGO) composites and antibodies for IL-6 sensing. The MES demonstrated excellent analytical performance for the simultaneous detection of UA and IL-6 in human blood, featuring high selectivity, broad linear ranges (10-1000 µM for UA and 0.001-100 ng/mL for IL-6), robust stability, and strong concordance with reference methods (R² > 0.95). This study provides a proof-of-concept platform for gout-related biomarker determination and offers a new strategy for the development of multi-biomarker electroanalytical devices.

Graphical Abstract