<p>Surface-enhanced Raman spectroscopy (SERS) is a rapid and sensitive analytical tool that has been widely applied in on-site food safety detection. However, challenges remain in improving the reproducibility, sensitivity, and portability of SERS detection. Recently, the integration of SERS with microfluidic analytical devices has attracted increasing attention because microfluidic devices enabled controllable sample handling and improved operational stability for on-site detection. Herein, compared with earlier review, this review emphasized recent progresses in integration strategies and device designs. First of all, we discussed typical approaches for combining SERS with microfluidic platforms, including label-free and label-based formats. Subsequently, the roles of microfluidic devices in SERS-microfluidic analytical devices were discussed. At last, the applications of SERS coupled with microfluidic devices in on-site food safety detection were summarized. Major challenges and future directions were outlined, including improving substrate reproducibility and scalability, minimizing material interference, enhancing robustness in complex food matrices, enabling multiplex and portable detection, and promoting standardization and validation for real-world deployment.</p> Graphical Abstract <p></p>