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<p>To enhance the therapeutic efficacy of breast cancer cells, a multifunctional nanoplatform was developed based on near-infrared AgInS<sub>2</sub> quantum dots (AIS QDs) and rolling circle amplification (RCA). The AIS QDs were synthesized via an environmentally benign hydrothermal method using glutathione as a surface ligand, demonstrating favorable optical and photothermal properties. The RCA-generated long DNA strands served as a scaffold to co-assemble AS1411 aptamer-conjugated AIS QDs, gene therapeutic agent (DNAzyme), and doxorubicin (Dox), forming a multimodal nanoplatform (AIS QDs@RD). The polyvalent aptamer sequence carried by AIS QDs@RD enabled specific recognition of the overexpressed nucleolin on the membrane of MCF-7 cells (human breast cancer cells), allowing rapid discrimination between cancer and normal cells. AIS QDs@RD had excellent performance, including optimal excitation/emission wavelengths at 470/650 nm, a high photoluminescence quantum yield (PL QY) of 58.72%, a photothermal conversion efficiency (<InlineEquation ID="IEq1"> <EquationSource Format="TEX">\(\eta\)</EquationSource> </InlineEquation>) of 37.74%, and a Dox loading efficiency of 80.53%, which could significantly improve fluorescence imaging and drug delivery. Cytotoxicity assays revealed that treatment with AIS QDs@RD for 48&#xa0;h, the viability of MCF-7 cells decreased to 31.8%, indicating potent antitumor activity, while minimal toxicity to normal cells. Therefore, this nanoplatform presents a promising strategy for targeted fluorescence imaging and therapy of breast cancer.</p> Graphical Abstract <p></p>

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Multifunctional DNA nanoplatform based on near-infrared AgInS2 QDs and rolling circle amplification for fluorescence imaging and therapy of breast cancer cells

  • 期刊论文

  • 发表日期：

    2026年3月22日

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  • Kexin Ding,
  • Jingyun Zhuang,
  • Gan Ning,
  • Tianxin Weng,
  • Xiufeng Wang,
  • Ting Zhou,
  • Fang Wang,
  • Zhiqing Zhang

摘要

To enhance the therapeutic efficacy of breast cancer cells, a multifunctional nanoplatform was developed based on near-infrared AgInS2 quantum dots (AIS QDs) and rolling circle amplification (RCA). The AIS QDs were synthesized via an environmentally benign hydrothermal method using glutathione as a surface ligand, demonstrating favorable optical and photothermal properties. The RCA-generated long DNA strands served as a scaffold to co-assemble AS1411 aptamer-conjugated AIS QDs, gene therapeutic agent (DNAzyme), and doxorubicin (Dox), forming a multimodal nanoplatform (AIS QDs@RD). The polyvalent aptamer sequence carried by AIS QDs@RD enabled specific recognition of the overexpressed nucleolin on the membrane of MCF-7 cells (human breast cancer cells), allowing rapid discrimination between cancer and normal cells. AIS QDs@RD had excellent performance, including optimal excitation/emission wavelengths at 470/650 nm, a high photoluminescence quantum yield (PL QY) of 58.72%, a photothermal conversion efficiency ( \(\eta\) ) of 37.74%, and a Dox loading efficiency of 80.53%, which could significantly improve fluorescence imaging and drug delivery. Cytotoxicity assays revealed that treatment with AIS QDs@RD for 48 h, the viability of MCF-7 cells decreased to 31.8%, indicating potent antitumor activity, while minimal toxicity to normal cells. Therefore, this nanoplatform presents a promising strategy for targeted fluorescence imaging and therapy of breast cancer.

Graphical Abstract