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<p>In-situ sterilization following detection is a challenge in microbiological detecting. Herein, we propose a dual-mode aptasensor for detecting <i>Pseudomonas aeruginosa</i> and achieving in-situ sterilization for the first time. Specifically, upon capture of <i>P. aeruginosa</i> by the histidine-modified aptamer-functionalized magnetic nanozyme (PA@HIS-MBs), the endogenous c-type cytochromes (c-cyts) of the bacterium and HIS-MBs established a dual peroxidase-like (POD-like) activities cascade system. H<sub>2</sub>O<sub>2</sub>-catalyzed reactive oxygen species (ROS) simultaneously oxidized TMB (3,3′,5,5′-tetramethylbenzidine) (colorimetric mode), in synergy with the photothermal effect of PA@HIS-MBs, achieved in-situ sterilization. Simultaneously, electrochemical impedance enabled accurate quantitative detection. Ultimately, the detection limits for the dual-mode aptasensor were 0.481&#xa0;CFU/mL (colorimetric mode) and 0.401&#xa0;CFU/mL (impedance mode), respectively. Furthermore, this aptasensor not only exhibited good matrix effect performance (|ME|&lt; 10%), but also delivered results consistent with quantitative polymerase chain reaction (qPCR) across three meat samples. It provided a universal platform for accurate, and rapid detection and in-situ sterilization of pathogenic bacteria, with potential for extension to other pathogenic bacteria.</p> Graphical abstract <p></p>

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A dual-mode aptasensor based on microbial endogenous cytochrome self-activated dual-enzyme cascade response for Pseudomonas aeruginosa detection and in-situ sterilization

  • 期刊论文

  • 发表日期：

    2026年3月21日

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  • Ziwei Song,
  • Wei Yuan,
  • Wenzhuo Wang,
  • Zhilan Sun,
  • Jiansheng Zhao,
  • Yanhong Tang,
  • Li Zhao,
  • Daoying Wang,
  • Fang Liu

摘要

In-situ sterilization following detection is a challenge in microbiological detecting. Herein, we propose a dual-mode aptasensor for detecting Pseudomonas aeruginosa and achieving in-situ sterilization for the first time. Specifically, upon capture of P. aeruginosa by the histidine-modified aptamer-functionalized magnetic nanozyme (PA@HIS-MBs), the endogenous c-type cytochromes (c-cyts) of the bacterium and HIS-MBs established a dual peroxidase-like (POD-like) activities cascade system. H2O2-catalyzed reactive oxygen species (ROS) simultaneously oxidized TMB (3,3′,5,5′-tetramethylbenzidine) (colorimetric mode), in synergy with the photothermal effect of PA@HIS-MBs, achieved in-situ sterilization. Simultaneously, electrochemical impedance enabled accurate quantitative detection. Ultimately, the detection limits for the dual-mode aptasensor were 0.481 CFU/mL (colorimetric mode) and 0.401 CFU/mL (impedance mode), respectively. Furthermore, this aptasensor not only exhibited good matrix effect performance (|ME|< 10%), but also delivered results consistent with quantitative polymerase chain reaction (qPCR) across three meat samples. It provided a universal platform for accurate, and rapid detection and in-situ sterilization of pathogenic bacteria, with potential for extension to other pathogenic bacteria.

Graphical abstract