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<p>A multi-walled carbon nanotube - poly (allylamine hydrochloride acid)/platinum nanoparticle (MWCNT-PAH/PtNP) biosensor for phosphorylated Tau-217 (pTau-217) detection and validated using Alzheimer’s disease (AD) plasma samples is described. Morphological characterization confirmed successful deposition of MWCNT-PAH/PtNP, which enhanced the sensor’s surface height range (bare sensor |∆| = 2.2&#xa0;μm vs. modified sensor |∆| = 5.7&#xa0;μm) and electrochemical properties. The sensor achieved a detection limit of 9.85 pg/mL by cyclic voltammetry (CV) and 3.83 pg/mL by chronoamperometry (CA), and could detect pTau-217 in the presence of interfering molecules and in complex protein-rich matrices such as fetal bovine serum (FBS). When validated on clinical samples, the sensor could distinguish AD from cognitive normal (CN )&#xa0;samples more effectively than ELISA (AUC<sub>CV</sub>: 90.75%; AUC<sub>CA</sub>: 87.13%; AUC<sub>ELISA</sub>: 52.52%). Subgroup analysis revealed that sensor output primarily reflected disease status, with minimal confounding effects from demographic or social factors. However, a key limitation is that current intensity cannot be directly converted into plasma pTau-217 concentration, restricting the sensor to qualitative AD screening rather than precise quantification. Taken together, this biosensor holds great potential as a cost-effective tool for distinguishing AD and CN plasma samples, with promising prospects for on-site application.</p> Graphical abstract <p></p>

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Development and human samples validation of a plasma pTau‑217 electrochemical sensor for Alzheimer’s detection in Vietnamese patients

  • 期刊论文

  • 发表日期：

    2026年2月19日

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  • Hoai Bao Pham,
  • Mai Trang Tong,
  • Nguyen Thuy Linh Tran,
  • Tien Tai Tran,
  • Thien Luan Phan,
  • Congo Tak Shing Ching,
  • Chan Khon Huynh,
  • Thi Thanh Huong Ha,
  • Cong Thang Tran

摘要

A multi-walled carbon nanotube - poly (allylamine hydrochloride acid)/platinum nanoparticle (MWCNT-PAH/PtNP) biosensor for phosphorylated Tau-217 (pTau-217) detection and validated using Alzheimer’s disease (AD) plasma samples is described. Morphological characterization confirmed successful deposition of MWCNT-PAH/PtNP, which enhanced the sensor’s surface height range (bare sensor |∆| = 2.2 μm vs. modified sensor |∆| = 5.7 μm) and electrochemical properties. The sensor achieved a detection limit of 9.85 pg/mL by cyclic voltammetry (CV) and 3.83 pg/mL by chronoamperometry (CA), and could detect pTau-217 in the presence of interfering molecules and in complex protein-rich matrices such as fetal bovine serum (FBS). When validated on clinical samples, the sensor could distinguish AD from cognitive normal (CN ) samples more effectively than ELISA (AUCCV: 90.75%; AUCCA: 87.13%; AUCELISA: 52.52%). Subgroup analysis revealed that sensor output primarily reflected disease status, with minimal confounding effects from demographic or social factors. However, a key limitation is that current intensity cannot be directly converted into plasma pTau-217 concentration, restricting the sensor to qualitative AD screening rather than precise quantification. Taken together, this biosensor holds great potential as a cost-effective tool for distinguishing AD and CN plasma samples, with promising prospects for on-site application.

Graphical abstract