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<p>Alzheimer’s disease (AD) is a prevalent neurodegenerative disorder characterized by the pathological aggregation of amyloid-β (Aβ) protein. In this study, a plasmon-enhanced fluorescence (PEF) sensor based on Ag nanocubes (Agcubes) was developed for in situ imaging and tracking of Aβ<sub>1−42</sub> (Aβ<sub>42</sub>) aggregation states. By precisely tuning the thickness of the silicon shell of the PEF sensor, the distance between rose bengals (RB) and Agcubes was optimized, leading to a remarkable 7.4-fold enhancement in fluorescence signal compared to RB. This sensor exhibited a significant “turn-on” response towards Aβ<sub>42</sub> aggregation, with an approximate 4.5-fold increase in fluorescence. Notably, the sensor could be successfully applied to detect and locate the process of Aβ<sub>42</sub> aggregation in living human neuroblastoma (SH-SY5Y) cells. Additionally, its staining sites were nearly identical to those of the commercial dye thioflavin T (ThT). This method enabled real-time in situ monitoring of intracellular Aβ<sub>42</sub> aggregation state, demonstrating great potential as a reliable diagnostic tool for AD.</p> Graphical abstract <p></p>

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Silver nanocubes-based plasmon-enhanced fluorescence for amplified detection and in situ tracking of amyloid-β aggregate

  • 期刊论文

  • 发表日期：

    2026年1月2日

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  • Chenye Xu,
  • Rong Xu,
  • Xiangyuan Yin,
  • Yaqiong Yang

摘要

Alzheimer’s disease (AD) is a prevalent neurodegenerative disorder characterized by the pathological aggregation of amyloid-β (Aβ) protein. In this study, a plasmon-enhanced fluorescence (PEF) sensor based on Ag nanocubes (Agcubes) was developed for in situ imaging and tracking of Aβ1−42 (Aβ42) aggregation states. By precisely tuning the thickness of the silicon shell of the PEF sensor, the distance between rose bengals (RB) and Agcubes was optimized, leading to a remarkable 7.4-fold enhancement in fluorescence signal compared to RB. This sensor exhibited a significant “turn-on” response towards Aβ42 aggregation, with an approximate 4.5-fold increase in fluorescence. Notably, the sensor could be successfully applied to detect and locate the process of Aβ42 aggregation in living human neuroblastoma (SH-SY5Y) cells. Additionally, its staining sites were nearly identical to those of the commercial dye thioflavin T (ThT). This method enabled real-time in situ monitoring of intracellular Aβ42 aggregation state, demonstrating great potential as a reliable diagnostic tool for AD.

Graphical abstract