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<p>The rational design of a photoactivated platinum–ferrocene-based metal–organic framework (Pt-Fc-MOF) biosensing platform is reported featuring triple-signal amplification for the rapid and visual detection of chloramphenicol (CAP) residues. The system combines aptamer-coated magnetic nanoparticles, cDNA-linked Pt-Fc-MOFs, and light-enhanced catalysis for triple-signal amplification. Upon specific target recognition, CAP binding induces aptamer conformational change, triggering the release of signal probes via nucleic acid strand displacement. The liberated Pt-Fc-MOFs incorporate ferrocene and platinum components that synergistically catalyze the oxidation of 3,3’,5,5’-tetramethylbenzidine (TMB) in the presence of H₂O₂, which is further enhanced upon light irradiation, generating an intensified colorimetric signal visible to the naked eye. This system demonstrates excellent sensitivity, with a remarkably low limit of detection (LOD) of 0.00016 ng·mL⁻¹, a wide linear detection range spanning from 0.0005 to 10 ng·mL⁻¹ and enables rapid, portable, and minimally instrumented detection. The incorporation of photoactivated catalysis offers a robust and portable strategy for real-time monitoring of foodborne antibiotics, advancing the development of next-generation MOF-based biosensors for point-of-care food safety diagnostics.</p> Graphical abstract <p></p>

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Photoactivated triple-signal amplification in engineered Pt-Fc-MOFs for rapid and visual detection of foodborne antibiotics

  • 期刊论文

  • 发表日期：

    2026年1月28日

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  • Hong-Xia Ren,
  • Xiao-Ting Liao,
  • Yi Xu,
  • Ming-Ying Wei,
  • Yang-Bao Miao

摘要

The rational design of a photoactivated platinum–ferrocene-based metal–organic framework (Pt-Fc-MOF) biosensing platform is reported featuring triple-signal amplification for the rapid and visual detection of chloramphenicol (CAP) residues. The system combines aptamer-coated magnetic nanoparticles, cDNA-linked Pt-Fc-MOFs, and light-enhanced catalysis for triple-signal amplification. Upon specific target recognition, CAP binding induces aptamer conformational change, triggering the release of signal probes via nucleic acid strand displacement. The liberated Pt-Fc-MOFs incorporate ferrocene and platinum components that synergistically catalyze the oxidation of 3,3’,5,5’-tetramethylbenzidine (TMB) in the presence of H₂O₂, which is further enhanced upon light irradiation, generating an intensified colorimetric signal visible to the naked eye. This system demonstrates excellent sensitivity, with a remarkably low limit of detection (LOD) of 0.00016 ng·mL⁻¹, a wide linear detection range spanning from 0.0005 to 10 ng·mL⁻¹ and enables rapid, portable, and minimally instrumented detection. The incorporation of photoactivated catalysis offers a robust and portable strategy for real-time monitoring of foodborne antibiotics, advancing the development of next-generation MOF-based biosensors for point-of-care food safety diagnostics.

Graphical abstract