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<p>A reusable magnetic enzyme-loaded carrier platform, Fe<sub>3</sub>O<sub>4</sub>/g-C<sub>3</sub>N<sub>4</sub>@CuMOF, synthesized by doping g-C<sub>3</sub>N<sub>4</sub> into CuMOF and then loading magnetic Fe<sub>3</sub>O<sub>4</sub>&#xa0;is reported. This carrier exhibits enhanced peroxidase-like activity, possesses broad-spectrum enzyme-loading capacity, and can load various natural enzymes to construct cascade sensors for colorimetric detection. Glucose oxidase, lactate oxidase, and galactose oxidase have been successfully loaded onto this carrier respectively, resulting in three cascade sensors with dual-enzyme activity that achieved sensitive colorimetric detection of glucose, lactic acid, and galactose. The detection limit is as low as 0.23 µM, with results consistent with authoritative methods. This carrier not only effectively preserves the activity of natural enzymes but also significantly improves their stability. Its magnetic property enables rapid separation of nanozyme from the test solution, eliminating light scattering interference and improving detection sensitivity. Moreover, this carrier exhibits excellent reusability. Such a high-performance enzyme-loaded carrier platform streamlines the construction of enzyme-nanozyme cascade systems and demonstrates great application potential.</p> Graphical Abstract <p></p>

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A versatile Fe3O4/g-C3N4@Cu-MOF magnetic platform with enhanced peroxidase-like activity for diverse enzymes immobilization and biomimetic cascade biosensing

  • 期刊论文

  • 发表日期：

    2025年11月21日

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  • Minnan Zhu,
  • Guangyan Li,
  • Xiu Cheng,
  • Yayang Tian,
  • Yuan Zhao,
  • Tao Chen,
  • Linfeng Chen

摘要

A reusable magnetic enzyme-loaded carrier platform, Fe3O4/g-C3N4@CuMOF, synthesized by doping g-C3N4 into CuMOF and then loading magnetic Fe3O4 is reported. This carrier exhibits enhanced peroxidase-like activity, possesses broad-spectrum enzyme-loading capacity, and can load various natural enzymes to construct cascade sensors for colorimetric detection. Glucose oxidase, lactate oxidase, and galactose oxidase have been successfully loaded onto this carrier respectively, resulting in three cascade sensors with dual-enzyme activity that achieved sensitive colorimetric detection of glucose, lactic acid, and galactose. The detection limit is as low as 0.23 µM, with results consistent with authoritative methods. This carrier not only effectively preserves the activity of natural enzymes but also significantly improves their stability. Its magnetic property enables rapid separation of nanozyme from the test solution, eliminating light scattering interference and improving detection sensitivity. Moreover, this carrier exhibits excellent reusability. Such a high-performance enzyme-loaded carrier platform streamlines the construction of enzyme-nanozyme cascade systems and demonstrates great application potential.

Graphical Abstract