Scholarlink

<p>A microfluidic-integrated electrochemical biosensing platform was developed, which uses two modified electrodes to separately detect two acute stroke&#xa0;(AS biomarkers) -&#xa0;homocysteine (Hcy) and adenosine (Ado) -&#xa0;from whole blood. The system integrates an on-chip plasma separator with multimodal electrochemical detection through rationally designed microchannels and nanostructure-based sensing interfaces. It enables the sensitive and selective quantification of Hcy and Ado in whole blood within 10&#xa0;min and 40&#xa0;min, respectively, with limits of detection (LOD) of 1.04 µM and 1.65 × 10⁻³ µM, respectively. The entire assay process for Ado requires a 30-minute incubation of the isolated plasma prior to differential pulse voltammetry (DPV) measurements. By performing automated sample-to-answer analysis, this platform provides a reliable method for the detection of AS-related biomarkers. Furthermore, the integration of on-chip blood processing with electrochemical detection presents a promising approach for future point-of-care and emergency diagnostic applications.</p> Graphical Abstract <p></p> <p> Schematic diagram of the integration and analysis procedure of the microfluidic electrochemical biosensing system.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

A sample-to-answer multimodal microfluidic integrated electrochemical biosensing system for rapid diagnosis of acute stroke from whole blood

  • 期刊论文

  • 发表日期：

    2025年11月15日

查看全文
  • Zehui Shi,
  • Zheng Gong,
  • Hongli Zhao,
  • Lin Zhou,
  • Hongju Mao,
  • Jianlong Zhao,
  • Minbo Lan

摘要

A microfluidic-integrated electrochemical biosensing platform was developed, which uses two modified electrodes to separately detect two acute stroke (AS biomarkers) - homocysteine (Hcy) and adenosine (Ado) - from whole blood. The system integrates an on-chip plasma separator with multimodal electrochemical detection through rationally designed microchannels and nanostructure-based sensing interfaces. It enables the sensitive and selective quantification of Hcy and Ado in whole blood within 10 min and 40 min, respectively, with limits of detection (LOD) of 1.04 µM and 1.65 × 10⁻³ µM, respectively. The entire assay process for Ado requires a 30-minute incubation of the isolated plasma prior to differential pulse voltammetry (DPV) measurements. By performing automated sample-to-answer analysis, this platform provides a reliable method for the detection of AS-related biomarkers. Furthermore, the integration of on-chip blood processing with electrochemical detection presents a promising approach for future point-of-care and emergency diagnostic applications.

Graphical Abstract

Schematic diagram of the integration and analysis procedure of the microfluidic electrochemical biosensing system.