Scholarlink

<p> A composite nanozyme (Ni<sub>3</sub>PMo<sub>12</sub>V<sub>2</sub>@SWNT) consisting of polyoxometalate-based metal-organic framework (POMOF) and single-walled carbon nanotubes (SWNTs) was prepared, and its oxidase-like activity was significantly enhanced by combining with ultrasonic (US) treatment. On one hand, the introduction of SWNTs increases catalytic active sites and constructs efficient electron (e⁻) transport channels; on the other hand, US reconstructs the Ni<sub>3</sub>PMo<sub>12</sub>V<sub>2</sub>@SWNT composite structure through mechanical dispersion and cavitation effects, promoting efficient electron transfer and improving catalytic activity. The highest catalytic activity of Ni<sub>3</sub>PMo<sub>12</sub>V<sub>2</sub>@SWNT-US was verified by calculating Michaelis-Menten kinetic parameters (K<sub>m</sub> and V<sub>max</sub>). Specifically, the order of catalytic activity is as follows: Ni<sub>3</sub>PMo<sub>12</sub>V<sub>2</sub>@SWNT-US (K<sub>m</sub>=0.0465 mM, V<sub>max</sub>=5.39 × 10⁻⁷ M·s⁻¹) &gt; Ni<sub>3</sub>PMo<sub>12</sub>V<sub>2</sub>@SWNT (K<sub>m</sub>=0.0513 mM, V<sub>max</sub>=3.75 × 10⁻⁷ M·s⁻¹) &gt; Ni<sub>3</sub>PMo<sub>12</sub>V<sub>2</sub> (K<sub>m</sub>=0.1004 mM, V<sub>max</sub>=3.18 × 10⁻⁷ M·s⁻¹). Based on this, a US-enhanced highly sensitive colorimetric detection system for glutathione (GSH) was constructed, which exhibits a linear detection range of 5–100 µM, a limit of detection of 0.055 µM, and a recovery of 99.7%–104.3% in simulated human serum, along with excellent stability and selectivity. This study provides a new feasible approach for optimizing the catalytic performance of nanozymes using ultrasonic technology and achieving efficient detection of small biological molecules.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Ultrasound-enhanced Ni3PMo12V2@SWNT nanocomposite for highly sensitive detection of glutathione

  • 期刊论文

  • 发表日期：

    2025年10月28日

查看全文
  • Jinhang Guo,
  • Lin Yin,
  • Yue Jiang,
  • Xu Yao,
  • Qinghui Zhao,
  • Bohui Cui,
  • Jingwen Sun,
  • Hong Zhao

摘要

A composite nanozyme (Ni3PMo12V2@SWNT) consisting of polyoxometalate-based metal-organic framework (POMOF) and single-walled carbon nanotubes (SWNTs) was prepared, and its oxidase-like activity was significantly enhanced by combining with ultrasonic (US) treatment. On one hand, the introduction of SWNTs increases catalytic active sites and constructs efficient electron (e⁻) transport channels; on the other hand, US reconstructs the Ni3PMo12V2@SWNT composite structure through mechanical dispersion and cavitation effects, promoting efficient electron transfer and improving catalytic activity. The highest catalytic activity of Ni3PMo12V2@SWNT-US was verified by calculating Michaelis-Menten kinetic parameters (Km and Vmax). Specifically, the order of catalytic activity is as follows: Ni3PMo12V2@SWNT-US (Km=0.0465 mM, Vmax=5.39 × 10⁻⁷ M·s⁻¹) > Ni3PMo12V2@SWNT (Km=0.0513 mM, Vmax=3.75 × 10⁻⁷ M·s⁻¹) > Ni3PMo12V2 (Km=0.1004 mM, Vmax=3.18 × 10⁻⁷ M·s⁻¹). Based on this, a US-enhanced highly sensitive colorimetric detection system for glutathione (GSH) was constructed, which exhibits a linear detection range of 5–100 µM, a limit of detection of 0.055 µM, and a recovery of 99.7%–104.3% in simulated human serum, along with excellent stability and selectivity. This study provides a new feasible approach for optimizing the catalytic performance of nanozymes using ultrasonic technology and achieving efficient detection of small biological molecules.

Graphical Abstract