Scholarlink

<p> A&#xa0;high-density microneedle (MN) electrochemical aptasensor integrated with gold nanoparticles (AuNPs) and aptamer recognition elements, enabling label-free, minimally invasive, and rapid detection of carcinoembryonic antigen (CEA) in interstitial fluid (ISF)&#xa0;is proposed. The AuNP-functionalized MNs enhance electrochemical signal transduction owing to their substantial electroactive surface area and high density of catalytic sites. Concurrently, CEA aptamers enable their covalent immobilization onto the nanostructured AuNP-modified electrode surface through gold-thiol (Au–S) bonds. Electrochemical analyses demonstrate a wide linear detection range (0.05–500&#xa0;ng/mL), low limits of detection (0.012&#xa0;ng/mL in phosphate buffer solution (PBS), 0.028&#xa0;ng/mL in artificial interstitial fluid (ISF)). The biosensor also exhibited excellent stability, repeatability, and specificity. As a proof of concept, skin model tests further confirmed the ability of the MN biosensor to extract and electrochemically detect CEA levels in artificial ISF, with a detection limit of 0.034&#xa0;ng/mL. This sampling-detection integrated biosensor provides a new approach for wearable point-of-care technologies in early cancer diagnosis.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

High-density AuNP-decorated microneedle electrochemical aptasensor for minimally invasive CEA detection in interstitial fluid

  • 期刊论文

  • 发表日期：

    2025年11月1日

查看全文
  • Ziwei Lian,
  • Tianlang Ou,
  • Kuo Men,
  • Qianhui Wei,
  • Feng Wei,
  • Hongbin Zhao,
  • Zhimin Yang,
  • Hailing Tu

摘要

A high-density microneedle (MN) electrochemical aptasensor integrated with gold nanoparticles (AuNPs) and aptamer recognition elements, enabling label-free, minimally invasive, and rapid detection of carcinoembryonic antigen (CEA) in interstitial fluid (ISF) is proposed. The AuNP-functionalized MNs enhance electrochemical signal transduction owing to their substantial electroactive surface area and high density of catalytic sites. Concurrently, CEA aptamers enable their covalent immobilization onto the nanostructured AuNP-modified electrode surface through gold-thiol (Au–S) bonds. Electrochemical analyses demonstrate a wide linear detection range (0.05–500 ng/mL), low limits of detection (0.012 ng/mL in phosphate buffer solution (PBS), 0.028 ng/mL in artificial interstitial fluid (ISF)). The biosensor also exhibited excellent stability, repeatability, and specificity. As a proof of concept, skin model tests further confirmed the ability of the MN biosensor to extract and electrochemically detect CEA levels in artificial ISF, with a detection limit of 0.034 ng/mL. This sampling-detection integrated biosensor provides a new approach for wearable point-of-care technologies in early cancer diagnosis.

Graphical Abstract