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<p> A&#xa0;dual-mode biosensing platform for the sensitive detection of <i>Listeria monocytogenes&#xa0;(L. monocytogenes)</i>&#xa0;is presented. This method integrated vancomycin-modified magnetic nanoparticles (MNPs, 10‒30&#xa0;nm) for efficient bacterial capture compared to <i>L. monocytogenes</i> monoclonal antibody, and aptamer-conjugated Fe-doped L-lysine-derived nanozyme (Fe@LS) for target recognition and signal amplification. The Fe@LS nanozyme exhibited pronounced peroxidase (POD)-like activity, catalyzing the oxidation of TMB with a Michaelis constant (<i>K</i><sub>m</sub>) of 0.63&#xa0;mM and a maximum reaction velocity (<i>V</i><sub>max</sub>) of 1.89 × 10<sup>−8</sup>&#xa0;M·s<sup>−1</sup>. Within this system, vancomycin-functionalized magnetic beads (Fe<sub>3</sub>O<sub>4</sub>@SiO<sub>2</sub>@NH<sub>2</sub>@van) enabled broad-spectrum binding to bacterial peptidoglycan, while the aptamer-modified Fe@LS (apt/Fe@LS) conferred the specific recognition of <i>L. monocytogenes</i>. Both fluorescence and colorimetric modes exhibited linear responses across a concentration range from 1.0 × 10<sup>2</sup> to 1.0 × 10<sup>8</sup>&#xa0;CFU/mL (<i>R</i><sup>2</sup> &gt; 0.99), yielding a wider detection range compared to that catalyzed by horseradish peroxidase (HRP). When applied to simulated food samples (lake water, milk, and vegetable), the platform demonstrated satisfactory recoveries (90.0‒109.0%) and low inter-assay variability (&lt; 8.0% RSD). The proposed dual-mode biosensing strategy offers a robust and reliable tool for the detection of <i>L. monocytogenes</i> in complex food environments.</p> Graphical Abstract <p></p>

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Synergizing nanozyme-catalyzed signal amplification with magnetic separation for sensitive dual-mode Listeria monocytogenes detection

  • 期刊论文

  • 发表日期：

    2025年10月13日

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  • Yongqi Wang,
  • Jiali Liao,
  • Jiajie Li,
  • Zhuqian Xiao,
  • Hongpeng Wang,
  • Jun Huang,
  • Linkai Yu

摘要

A dual-mode biosensing platform for the sensitive detection of Listeria monocytogenes (L. monocytogenes) is presented. This method integrated vancomycin-modified magnetic nanoparticles (MNPs, 10‒30 nm) for efficient bacterial capture compared to L. monocytogenes monoclonal antibody, and aptamer-conjugated Fe-doped L-lysine-derived nanozyme (Fe@LS) for target recognition and signal amplification. The Fe@LS nanozyme exhibited pronounced peroxidase (POD)-like activity, catalyzing the oxidation of TMB with a Michaelis constant (Km) of 0.63 mM and a maximum reaction velocity (Vmax) of 1.89 × 10−8 M·s−1. Within this system, vancomycin-functionalized magnetic beads (Fe3O4@SiO2@NH2@van) enabled broad-spectrum binding to bacterial peptidoglycan, while the aptamer-modified Fe@LS (apt/Fe@LS) conferred the specific recognition of L. monocytogenes. Both fluorescence and colorimetric modes exhibited linear responses across a concentration range from 1.0 × 102 to 1.0 × 108 CFU/mL (R2 > 0.99), yielding a wider detection range compared to that catalyzed by horseradish peroxidase (HRP). When applied to simulated food samples (lake water, milk, and vegetable), the platform demonstrated satisfactory recoveries (90.0‒109.0%) and low inter-assay variability (< 8.0% RSD). The proposed dual-mode biosensing strategy offers a robust and reliable tool for the detection of L. monocytogenes in complex food environments.

Graphical Abstract