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<p>miRNA-21-5p and miRNA-155-5p have emerged as two promising biomarkers in body fluids during the early stages of lung cancer. This study focused on designing a dual-target optical biosensor for the simultaneous detection of miRNA-21 and miRNA-155. DNA hairpin probes and metal nanoclusters are the materials used in this design. Light-emitting gold nanoclusters (AuNCs) and copper-silver nanoclusters (Cu/AgNCs) were located on hairpin probes to achieve fluorescence-based detection. The biosensor leverages the distinct fluorescence properties of these nanoclusters to detect target miRNAs (miRNA-21 enhances the emission of Cu/AgNCs, while miRNA-155 quenches the emission of AuNCs). The biosensor demonstrated remarkable sensitivity toward miRNA-21 and miRNA-155. Detection limits were 1&#xa0;pM and 5&#xa0;pM, and linear response ranges were 15–140&#xa0;pM and 15–125&#xa0;pM for miRNA-21 and miRNA-155, respectively. The response of the nanobiosensor was validated by RT-qPCR as the gold standard method in analyses of miRNAs in human serum samples and lung cancer cells. The obtained results confirmed the accuracy and reliability of the biosensor. Compared to existing biosensors, the proposed design avoids complex surface modifications and enzyme usage, ensuring cost-effectiveness and operational simplicity.</p> Graphical abstract <p></p>

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Simultaneous recognition of two MicroRNAs associated with early stages of lung cancer by DNA hairpin-templated fluorescent nanoclusters

  • 期刊论文

  • 发表日期：

    2025年10月30日

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  • Sheida Zoughi,
  • Farnoush Faridbod,
  • Saeed Mohebbi,
  • Sharif Moradi

摘要

miRNA-21-5p and miRNA-155-5p have emerged as two promising biomarkers in body fluids during the early stages of lung cancer. This study focused on designing a dual-target optical biosensor for the simultaneous detection of miRNA-21 and miRNA-155. DNA hairpin probes and metal nanoclusters are the materials used in this design. Light-emitting gold nanoclusters (AuNCs) and copper-silver nanoclusters (Cu/AgNCs) were located on hairpin probes to achieve fluorescence-based detection. The biosensor leverages the distinct fluorescence properties of these nanoclusters to detect target miRNAs (miRNA-21 enhances the emission of Cu/AgNCs, while miRNA-155 quenches the emission of AuNCs). The biosensor demonstrated remarkable sensitivity toward miRNA-21 and miRNA-155. Detection limits were 1 pM and 5 pM, and linear response ranges were 15–140 pM and 15–125 pM for miRNA-21 and miRNA-155, respectively. The response of the nanobiosensor was validated by RT-qPCR as the gold standard method in analyses of miRNAs in human serum samples and lung cancer cells. The obtained results confirmed the accuracy and reliability of the biosensor. Compared to existing biosensors, the proposed design avoids complex surface modifications and enzyme usage, ensuring cost-effectiveness and operational simplicity.

Graphical abstract