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<p> A&#xa0;PtAu@Rh nanozyme with strong peroxidase (POD)-like activity was synthesized via the template method, and a colorimetric sensing platform for ascorbic acid (AA) detection based on UV‒vis or smartphone assistance was established. The results revealed that the specific activity of the PtAu@Rh-2 nanozyme reached 1789 U mg<sup>−1</sup>, with V<sub>max</sub> and K<sub>m</sub> of 1.3488 × 10<sup>−6</sup>&#xa0;M&#xa0;s<sup>−1</sup> and 1.8286&#xa0;mM, respectively. Under optimal detection conditions, the developed smartphone-assisted method achieved a wide detection range (0.5–50&#xa0;μM) and a low limit of detection (LOD, 0.138&#xa0;μM). Spike recovery experiments in various actual samples verified the reliability and feasibility of the developed method, yielding recoveries of 95.34% to 104.28% and relative standard deviations of 0.29% to 5.06%, both within the acceptable standard range. This work provides not only a reliable strategy for synthesizing high-efficiency nanozymes but also a convenient AA detection method to help individuals make reasonable and healthy dietary choices.</p> Graphical Abstract <p></p>

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A smartphone-integrated portable colorimetric sensing platform for ascorbic acid based on a PtAu@Rh nanozyme with high specific activity

  • 期刊论文

  • 发表日期：

    2025年9月16日

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  • Jian Hao,
  • Xiaojie Dong,
  • Zeyang Yu,
  • Kai Cai

摘要

A PtAu@Rh nanozyme with strong peroxidase (POD)-like activity was synthesized via the template method, and a colorimetric sensing platform for ascorbic acid (AA) detection based on UV‒vis or smartphone assistance was established. The results revealed that the specific activity of the PtAu@Rh-2 nanozyme reached 1789 U mg−1, with Vmax and Km of 1.3488 × 10−6 M s−1 and 1.8286 mM, respectively. Under optimal detection conditions, the developed smartphone-assisted method achieved a wide detection range (0.5–50 μM) and a low limit of detection (LOD, 0.138 μM). Spike recovery experiments in various actual samples verified the reliability and feasibility of the developed method, yielding recoveries of 95.34% to 104.28% and relative standard deviations of 0.29% to 5.06%, both within the acceptable standard range. This work provides not only a reliable strategy for synthesizing high-efficiency nanozymes but also a convenient AA detection method to help individuals make reasonable and healthy dietary choices.

Graphical Abstract