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<p>A sandwich-type electrochemical (EC) immunosensor for neuron-specific enolase (NSE) detection was constructed utilizing a signal amplification strategy. This strategy was implemented by utilizing palladium decorated CoS<sub>2</sub> hollow nanoboxes (CoS<sub>2</sub>-Pd HNBs) as the signal indicator and gold-functionalized Fe<sub>3</sub>O<sub>4</sub> (Fe<sub>3</sub>O<sub>4</sub>-Au) as the EC sensing platform. CoS<sub>2</sub> HNBs with hollow structures were synthesized via template method, which exhibited excellent electrocatalytic performance toward H<sub>2</sub>O<sub>2</sub>. Moreover, Pd nanoparticles (NPs) were decorated on CoS<sub>2</sub> HNBs by in situ reduction method, which further improved the electrocatalytic performance for H<sub>2</sub>O<sub>2</sub> reduction due to the synergetic catalysis. Modifying Au NPs uniformly on Fe<sub>3</sub>O<sub>4</sub> surface could not only enhance the electroconductivity of the EC sensing platform but also immobilize the capture antibody (Ab<sub>1</sub>) via strong coordination. Fe<sub>3</sub>O<sub>4</sub>-Au nanocomposites were anchored to the magnetic glass carbon electrode because of good magnetic properties, improving stability of the EC immunosensor. Prominently, the well-established sandwich-type EC immunosensor with NSE as a targeted detection object exhibited a low detection limit of 0.29&#xa0;pg/mL (S/N = 3) and a linear range from 500&#xa0;fg/mL to 50&#xa0;ng/mL. This work may provide a promising detection method for other biomarkers.</p> Graphical abstract <p></p>

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A signal-amplifying electrochemical immunosensor based on palladium decorated CoS2 hollow nanoboxes and gold-functionalized Fe3O4 for neuron-specific enolase detection

  • 期刊论文

  • 发表日期：

    2025年9月20日

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  • Dehao Jia,
  • Yujian Guo,
  • Lei Yang,
  • Xing Gao,
  • Dongquan Leng,
  • Qin Wei

摘要

A sandwich-type electrochemical (EC) immunosensor for neuron-specific enolase (NSE) detection was constructed utilizing a signal amplification strategy. This strategy was implemented by utilizing palladium decorated CoS2 hollow nanoboxes (CoS2-Pd HNBs) as the signal indicator and gold-functionalized Fe3O4 (Fe3O4-Au) as the EC sensing platform. CoS2 HNBs with hollow structures were synthesized via template method, which exhibited excellent electrocatalytic performance toward H2O2. Moreover, Pd nanoparticles (NPs) were decorated on CoS2 HNBs by in situ reduction method, which further improved the electrocatalytic performance for H2O2 reduction due to the synergetic catalysis. Modifying Au NPs uniformly on Fe3O4 surface could not only enhance the electroconductivity of the EC sensing platform but also immobilize the capture antibody (Ab1) via strong coordination. Fe3O4-Au nanocomposites were anchored to the magnetic glass carbon electrode because of good magnetic properties, improving stability of the EC immunosensor. Prominently, the well-established sandwich-type EC immunosensor with NSE as a targeted detection object exhibited a low detection limit of 0.29 pg/mL (S/N = 3) and a linear range from 500 fg/mL to 50 ng/mL. This work may provide a promising detection method for other biomarkers.

Graphical abstract