Scholarlink

<p>A dual-mode aptasensor was engineered for aflatoxin B<sub>1</sub> (AFB<sub>1</sub>) detection by functional integration of peroxidase-mimetic Au@CeO<sub>2</sub> core–shell nanostructures with emissive carbon dots (CDs). The Au@CeO<sub>2</sub> nanocomposite, synthesized via spontaneous redox&#xa0;reaction, exhibited enhanced peroxidase-like activity due to abundant Ce<sup>3+</sup>/oxygen vacancies facilitating hydroxyl radical generation. The aptasensor utilizes a competitive binding mechanism, where AFB<sub>1</sub> competed with immobilized Au@CeO<sub>2</sub>-CDs-Apt1 probes for binding sites, resulting in inversely proportional colorimetric and fluorescent signals. Under optimized conditions, the biosensor achieved a broad linear detection range (0.001–50&#xa0;ng/mL) with an ultralow limit of detection (LOD) of 0.0005&#xa0;ng/mL. The sensor demonstrated excellent selectivity, stability, and reproducibility, and was successfully applied to detect AFB<sub>1</sub> in spiked peanut samples, with recoveries ranging from 98.7 to 116.7%. This work not only advances the design of nanozyme-based biosensors but also provides a rapid, accurate, and field-deployable strategy for monitoring mycotoxins in complex food matrices.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

A colorimetric fluorometric dual-mode aptasensor for aflatoxin B1 detection driven by Au@CeO2 core–shell nanozymes

  • 期刊论文

  • 发表日期：

    2025年9月5日

查看全文
  • Ziyue Chen,
  • Lei Bai,
  • Xinhua Xie,
  • Xinhao Zhang,
  • Shuaiqi Wen,
  • Jieqiong Qin,
  • Shichang Zhang,
  • Bobo Zhang,
  • Hongshuai Zhu

摘要

A dual-mode aptasensor was engineered for aflatoxin B1 (AFB1) detection by functional integration of peroxidase-mimetic Au@CeO2 core–shell nanostructures with emissive carbon dots (CDs). The Au@CeO2 nanocomposite, synthesized via spontaneous redox reaction, exhibited enhanced peroxidase-like activity due to abundant Ce3+/oxygen vacancies facilitating hydroxyl radical generation. The aptasensor utilizes a competitive binding mechanism, where AFB1 competed with immobilized Au@CeO2-CDs-Apt1 probes for binding sites, resulting in inversely proportional colorimetric and fluorescent signals. Under optimized conditions, the biosensor achieved a broad linear detection range (0.001–50 ng/mL) with an ultralow limit of detection (LOD) of 0.0005 ng/mL. The sensor demonstrated excellent selectivity, stability, and reproducibility, and was successfully applied to detect AFB1 in spiked peanut samples, with recoveries ranging from 98.7 to 116.7%. This work not only advances the design of nanozyme-based biosensors but also provides a rapid, accurate, and field-deployable strategy for monitoring mycotoxins in complex food matrices.

Graphical Abstract