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<p>A high-affinity aptamer was developed using a molecular docking approach, leading to the establishment of an electrochemical aptamer biosensor for the detection of malachite green&#xa0;(MG). The aptamer designated as MG-15 was derived from a rational truncation of MG-36, yielding a dissociation constant (K<sub>d</sub>) of 45.4 <InlineEquation ID="IEq1"> <InlineMediaObject> <ImageObject Color="BlackWhite" FileRef="604_2025_7506_Article_IEq1.gif" Format="GIF" Height="13" Rendition="HTML" Resolution="72" Type="Linedraw" Width="19" /> </InlineMediaObject> <EquationSource Format="TEX">\(\pm\)</EquationSource> <EquationSource Format="MATHML"><math> <mo>±</mo> </math></EquationSource> </InlineEquation> 17.6&#xa0;μM. Further enhancements were achieved by completely mutating the binding site of MG-15, resulting in a higher affinity aptamer (MG-15-GGT) with a lower K<sub>d</sub> of 2.71 <InlineEquation ID="IEq2"> <InlineMediaObject> <ImageObject Color="BlackWhite" FileRef="604_2025_7506_Article_IEq1.gif" Format="GIF" Height="13" Rendition="HTML" Resolution="72" Type="Linedraw" Width="19" /> </InlineMediaObject> <EquationSource Format="TEX">\(\pm\)</EquationSource> <EquationSource Format="MATHML"><math> <mo>±</mo> </math></EquationSource> </InlineEquation> 1.95&#xa0;μM. This sensor exhibited a limit of detection (LOD) value of 2.74&#xa0;pM and demonstrated excellent specificity, with recoveries ranging from 87.1 to 111.5%. Consequently, electrochemical biosensors utilizing modified aptamers hold great promise for applications in food safety.</p> Graphical Abstract <p></p>

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Detection of malachite green by electrochemical sensor constructed from modified high-affinity aptamer

  • 期刊论文

  • 发表日期：

    2025年9月21日

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  • Zanlin Chen,
  • Haiming Li,
  • Yian Chen,
  • Shuangyan Han

摘要

A high-affinity aptamer was developed using a molecular docking approach, leading to the establishment of an electrochemical aptamer biosensor for the detection of malachite green (MG). The aptamer designated as MG-15 was derived from a rational truncation of MG-36, yielding a dissociation constant (Kd) of 45.4 \(\pm\) ± 17.6 μM. Further enhancements were achieved by completely mutating the binding site of MG-15, resulting in a higher affinity aptamer (MG-15-GGT) with a lower Kd of 2.71 \(\pm\) ± 1.95 μM. This sensor exhibited a limit of detection (LOD) value of 2.74 pM and demonstrated excellent specificity, with recoveries ranging from 87.1 to 111.5%. Consequently, electrochemical biosensors utilizing modified aptamers hold great promise for applications in food safety.

Graphical Abstract