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<p><i>Helicobacter pylori</i> (<i>H. pylori</i>) is a major pathogen associated with gastric ulcers and gastric cancer, infecting approximately half of the global population. Therefore, developing sensitive, rapid, and cost-effective detection methods for <i>H. pylori</i> is critical for public health. We developed an innovative microscale colorimetric microsensor integrated with smartphone ambient-light sensors for portable, on-site quantification of <i>H. pylori-</i>associated urease activity in dental plaque. This low-cost device (&lt; $&#xa0;5 fabrication) employs 3D-printed components and a custom app to measure transmittance changes from urease-catalyzed urea hydrolysis with phenol red, achieving a robust correlation with commercial microplate reader absorbance (<i>R</i><sup>2</sup> = 0.9989). The system demonstrates exceptional Analytical sensitivity, with limits of detection of 9.75 U/L for urease And 1.709 × 10<sup>3</sup>&#xa0;CFU/mL for <i>H. pylori</i>, alongside high reproducibility (CV &lt; 10%) And stability over 14&#xa0;days. When applied to the on-site detection of dental plaque among volunteers, the smartphone-based colorimetric microsensor exhibited detection results that were highly aligned with <i>H. pylori</i> infections confirmed by the urea breath test. It outperformed commercial test strips And achieved 100% sensitivity, 95.5% specificity, And 97.1% accuracy. This miniaturized, smartphone-based platform offers a promising solution for home self-testing and demonstrates significant potential for enhancing <i>H. pylori</i> preliminary screening at the community level.</p> Graphical abstract <p></p>

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Smartphone-based colorimetric detection of urease activity in dental plaque for point-of-care screening of Helicobacter pylori infection

  • 期刊论文

  • 发表日期：

    2025年9月13日

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  • Yuhan Wang,
  • Ping Zhang,
  • Chuxian Zhou,
  • Zicong Zheng,
  • Qiang Quan,
  • Guangbo He,
  • Hening Zhai,
  • Qifang Song,
  • Xiaoli Zhang,
  • Heng Li,
  • Lanhuan Peng,
  • Yuqin Liao,
  • Wei Fang,
  • Minmin Li,
  • Jiajie Liang,
  • Yong Tang

摘要

Helicobacter pylori (H. pylori) is a major pathogen associated with gastric ulcers and gastric cancer, infecting approximately half of the global population. Therefore, developing sensitive, rapid, and cost-effective detection methods for H. pylori is critical for public health. We developed an innovative microscale colorimetric microsensor integrated with smartphone ambient-light sensors for portable, on-site quantification of H. pylori-associated urease activity in dental plaque. This low-cost device (< $ 5 fabrication) employs 3D-printed components and a custom app to measure transmittance changes from urease-catalyzed urea hydrolysis with phenol red, achieving a robust correlation with commercial microplate reader absorbance (R2 = 0.9989). The system demonstrates exceptional Analytical sensitivity, with limits of detection of 9.75 U/L for urease And 1.709 × 103 CFU/mL for H. pylori, alongside high reproducibility (CV < 10%) And stability over 14 days. When applied to the on-site detection of dental plaque among volunteers, the smartphone-based colorimetric microsensor exhibited detection results that were highly aligned with H. pylori infections confirmed by the urea breath test. It outperformed commercial test strips And achieved 100% sensitivity, 95.5% specificity, And 97.1% accuracy. This miniaturized, smartphone-based platform offers a promising solution for home self-testing and demonstrates significant potential for enhancing H. pylori preliminary screening at the community level.

Graphical abstract