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<p>A novel fluorescent organic compound, OBEI, exhibiting aggregation-induced emission enhancement (AIEE) characteristics was successfully synthesized. Leveraging its unique photophysical properties, a label-free fluorescent aptasensor was developed&#xa0;using OBEI as the fluorescent reporter and Fe<sub>3</sub>O<sub>4</sub>@SiO<sub>2</sub> magnetic beads as the adsorption substrate. The sensing mechanism relies on the formation of an INS-Apt/OBEI complex through electrostatic and hydrophobic interactions between OBEI and the insulin-specific aptamer (INS-Apt). This complex becomes immobilized on the magnetic bead surface via hydrogen bonding. Upon insulin introduction, the strong target-aptamer affinity triggers complex dissociation from the magnetic substrate, leaving the fluorescent INS-Apt/OBEI complex in solution after magnetic separation. The fluorescent intensity of the supernatant exhibited a linear response to insulin concentrations ranging from 0.033&#xa0;pM to 10&#xa0;nM, achieving a remarkable detection limit of 0.01&#xa0;pM (S/N = 3). The biosensor demonstrates remarkable specificity for insulin against potential interferents and has been successfully implemented for insulin determination in human serum samples.</p> Graphical Abstract <p></p>

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Ultrasensitive determination of insulin by an unlabelled fluorescent aptasensor based on Fe3O4@SiO2 and an oxadiazole compound with AIEE activity

  • 期刊论文

  • 发表日期：

    2025年8月11日

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  • Zhifeng Li,
  • Yiwen Yang,
  • Qiukai Tang,
  • Yanbo Zeng,
  • Hailong Wang,
  • Lei Li

摘要

A novel fluorescent organic compound, OBEI, exhibiting aggregation-induced emission enhancement (AIEE) characteristics was successfully synthesized. Leveraging its unique photophysical properties, a label-free fluorescent aptasensor was developed using OBEI as the fluorescent reporter and Fe3O4@SiO2 magnetic beads as the adsorption substrate. The sensing mechanism relies on the formation of an INS-Apt/OBEI complex through electrostatic and hydrophobic interactions between OBEI and the insulin-specific aptamer (INS-Apt). This complex becomes immobilized on the magnetic bead surface via hydrogen bonding. Upon insulin introduction, the strong target-aptamer affinity triggers complex dissociation from the magnetic substrate, leaving the fluorescent INS-Apt/OBEI complex in solution after magnetic separation. The fluorescent intensity of the supernatant exhibited a linear response to insulin concentrations ranging from 0.033 pM to 10 nM, achieving a remarkable detection limit of 0.01 pM (S/N = 3). The biosensor demonstrates remarkable specificity for insulin against potential interferents and has been successfully implemented for insulin determination in human serum samples.

Graphical Abstract