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<p>A&#xa0;smartphone-integrated colorimetric sensing platform based on bovine serum albumin (BSA)-templated manganese dioxide (BSA/MnO<sub>2</sub>) nanoprobe was successfully constructed to achieve high sensitivity and high selectivity for the detection of azodicarbonamide&#xa0;(ADA) in flour. The BSA/MnO<sub>2&#xa0;</sub>nanoprobe was synthesized by a simple one-pot reduction method of BSA using KMnO<sub>4</sub>, without the need for complex post-processing, and had excellent water dispersibility. It undergoes redox reactions with glutathione (GSH) causing&#xa0;self-degradation. Since ADA can oxidize the thiol groups of GSH to disulfide bonds, thereby reducing GSH-mediated MnO<sub>2</sub> degradation, this property was cleverly used for the quantitative detection of ADA. By combining the colorimetric nanoprobe system with smartphone RGB analysis technology, we successfully developed a portable detection platform for accurate determination of ADA content&#xa0;in flour. This research not only provides a simple and practical nanoprobe detection solution for food safety monitoring, but also promotes the innovative application of smartphone-assisted detection technology in the field of food contaminant analysis.</p> Graphical Abstract <p></p>

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Smartphone-integrated colorimetric nanosensor for azodicarbonamide detection in flour using BSA/MnO₂ nanoprobes

  • 期刊论文

  • 发表日期：

    2025年8月19日

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  • Xiaoqing Yang,
  • Xuehong Min,
  • Xinru Nan,
  • Man Zhou,
  • Ziyue Gong,
  • Chendi Qin,
  • Yuxin Liao,
  • Xinrong Cheng,
  • Lei Qiao,
  • Xudong Wang,
  • Xiaoqing Yi

摘要

A smartphone-integrated colorimetric sensing platform based on bovine serum albumin (BSA)-templated manganese dioxide (BSA/MnO2) nanoprobe was successfully constructed to achieve high sensitivity and high selectivity for the detection of azodicarbonamide (ADA) in flour. The BSA/MnO2 nanoprobe was synthesized by a simple one-pot reduction method of BSA using KMnO4, without the need for complex post-processing, and had excellent water dispersibility. It undergoes redox reactions with glutathione (GSH) causing self-degradation. Since ADA can oxidize the thiol groups of GSH to disulfide bonds, thereby reducing GSH-mediated MnO2 degradation, this property was cleverly used for the quantitative detection of ADA. By combining the colorimetric nanoprobe system with smartphone RGB analysis technology, we successfully developed a portable detection platform for accurate determination of ADA content in flour. This research not only provides a simple and practical nanoprobe detection solution for food safety monitoring, but also promotes the innovative application of smartphone-assisted detection technology in the field of food contaminant analysis.

Graphical Abstract