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<p>An&#xa0;electrochemical immunosensor was&#xa0;successfully developed&#xa0;for evaluating traditional Chinese medicine&#xa0;(TCM)-induced cardiac toxicity by immobilizing heart-type fatty acid-binding protein&#xa0;(HFABP)-specific antibodies on an electrode surface modified with chitosan (CS)-functionalized metal–organic framework ZIF-8 and gold nanoparticles (AuNPs). Leveraging the excellent biocompatibility of AuNPs, the excellent stability and high surface area of ZIF-8, along with the strong specific recognition capability of HFABP antibodies, the sensor demonstrated superior performance. Under optimized experimental conditions, it achieved an ultra-low detection limit of 7.09 × 10<sup>−2</sup>&#xa0;pg&#xa0;mL<sup>−1</sup> for HFABP, maintained excellent linearity in the range 1.57 × 10<sup>−2</sup> ~ 1.60 × 10<sup>1</sup>&#xa0;ng&#xa0;mL<sup>−1</sup>, and outperformed conventional ELISA methods in sensitivity when applied to TCM-induced cardiac toxicity models. The sensor also demonstrated excellent specificity, reproducibility, and stability. This research provides a sensitive and rapid new method for early warning of TCM-induced cardiac toxicity and contributes technical support and scientific reference for improving diagnostic accuracy in herbal medicine safety assessment.</p> Graphical Abstract <p></p>

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An electrochemical immunosensor based on HFABP for the early detection of drug-induced cardiac toxicity from traditional Chinese medicines

  • 期刊论文

  • 发表日期：

    2025年9月3日

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  • Bolu Sun,
  • Jiali Kang,
  • Zixia Wang,
  • Yuqiong Wu,
  • Haiying He,
  • Xinlan Wang,
  • Xuanxiu Da,
  • Miao Zhou,
  • Ying Lv,
  • Chenyu Qin,
  • Xiangdong Wang,
  • Lin Yang,
  • Huajie Li,
  • Hai Wang

摘要

An electrochemical immunosensor was successfully developed for evaluating traditional Chinese medicine (TCM)-induced cardiac toxicity by immobilizing heart-type fatty acid-binding protein (HFABP)-specific antibodies on an electrode surface modified with chitosan (CS)-functionalized metal–organic framework ZIF-8 and gold nanoparticles (AuNPs). Leveraging the excellent biocompatibility of AuNPs, the excellent stability and high surface area of ZIF-8, along with the strong specific recognition capability of HFABP antibodies, the sensor demonstrated superior performance. Under optimized experimental conditions, it achieved an ultra-low detection limit of 7.09 × 10−2 pg mL−1 for HFABP, maintained excellent linearity in the range 1.57 × 10−2 ~ 1.60 × 101 ng mL−1, and outperformed conventional ELISA methods in sensitivity when applied to TCM-induced cardiac toxicity models. The sensor also demonstrated excellent specificity, reproducibility, and stability. This research provides a sensitive and rapid new method for early warning of TCM-induced cardiac toxicity and contributes technical support and scientific reference for improving diagnostic accuracy in herbal medicine safety assessment.

Graphical Abstract