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<p>Two selective methods were developed for detecting mercury ions (Hg<sup>2</sup>⁺) in food and water samples using catalytic fluorometry based on Au-Hg amalgamation with enhanced peroxidase-like activity. These approaches include (i) a laser-printed microfluidic paper-based analytical device (LP-µPAD) and (ii) a paper-based microcentrifuge tube test kit. The LP-µPAD was fabricated via commercial laser printing and integrated gold nanoparticles (AuNPs) with o-phenylenediamine (OPD) and hydrogen peroxide (H₂O₂). The test kit enabled Hg<sup>2</sup>⁺ detection in food by reducing Hg<sup>2</sup>⁺ to Hg⁰ with stannous chloride, facilitating Au-Hg amalgam formation in the detection zone. Both approaches utilized the Au-Hg amalgam to catalyze H₂O₂-mediated oxidation of OPD, generating fluorescent 2,3-diaminophenazine (DAP), further enhancing fluorescence intensity in proportion to Hg<sup>2</sup>⁺ concentrations. The LP-µPAD exhibited a detection range of 3.0–20.0&#xa0;µg L⁻<sup>1</sup> with a limit of detection (LOD) of 1.65&#xa0;µg L⁻<sup>1</sup>, whereas the test kit provided a detection range of 0.1–1.0&#xa0;mg L⁻<sup>1</sup> with an LOD of 0.08&#xa0;mg L⁻<sup>1</sup>. Both sensors showed high selectivity for Hg<sup>2</sup>⁺ over other ions and performed well in real sample analyses, aligning closely with results from conventional methods.</p> Graphical Abstract <p></p>

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Two approaches for mercury determination in environmental and food monitoring via catalytic fluorometry of Au-Hg amalgamation using a laser-printed paper sensor and microcentrifuge tube test kit

  • 期刊论文

  • 发表日期：

    2025年8月6日

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  • Nutthaporn Malahom,
  • Sukanya Boonthod,
  • Nattapong Veerasup,
  • Chaiwat Pajarean,
  • Akarapong Prakobkij,
  • Wipark Anutrasakda,
  • Daniel Citterio,
  • Maliwan Amatatongchai,
  • Duangjai Nacapricha,
  • Purim Jarujamrus

摘要

Two selective methods were developed for detecting mercury ions (Hg2⁺) in food and water samples using catalytic fluorometry based on Au-Hg amalgamation with enhanced peroxidase-like activity. These approaches include (i) a laser-printed microfluidic paper-based analytical device (LP-µPAD) and (ii) a paper-based microcentrifuge tube test kit. The LP-µPAD was fabricated via commercial laser printing and integrated gold nanoparticles (AuNPs) with o-phenylenediamine (OPD) and hydrogen peroxide (H₂O₂). The test kit enabled Hg2⁺ detection in food by reducing Hg2⁺ to Hg⁰ with stannous chloride, facilitating Au-Hg amalgam formation in the detection zone. Both approaches utilized the Au-Hg amalgam to catalyze H₂O₂-mediated oxidation of OPD, generating fluorescent 2,3-diaminophenazine (DAP), further enhancing fluorescence intensity in proportion to Hg2⁺ concentrations. The LP-µPAD exhibited a detection range of 3.0–20.0 µg L⁻1 with a limit of detection (LOD) of 1.65 µg L⁻1, whereas the test kit provided a detection range of 0.1–1.0 mg L⁻1 with an LOD of 0.08 mg L⁻1. Both sensors showed high selectivity for Hg2⁺ over other ions and performed well in real sample analyses, aligning closely with results from conventional methods.

Graphical Abstract