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<p>An&#xa0;electrochemical aptasensor for hydrocortisone (HC) detection was developed&#xa0;using a vanadium-based porphyrinic metal–organic framework (V-PMOF) materials synthesized from vanadium-based MXene (V₂CTₓ) and tetrakis (4-carboxyphenyl) porphyrin (TCPP), followed by thermal treatment at 800&#xa0;°C to obtain V-PMOF<sub>800°C</sub>. The resulting V-PMOF<sub>800°C</sub> retained a nanoflower-like porous structure, enhancing electron transport, structural integrity, and electroactive site accessibility. Gold nanoparticles (Au NPs) were electrodeposited onto the V-PMOF<sub>800°C</sub>-modified electrode to improve conductivity and enable thiolated DNA aptamer (DNA<sub>apt</sub>) immobilization via Au–S bonding. Electrochemical measurements, particularly differential pulse voltammetry (DPV), were employed to evaluate the performance of the aptasensor, which exhibited a wide linear concentration range (0.05&#xa0;nM–5&#xa0;μM) and a low detection limit (LOD) of 10&#xa0;pM. This work introduces a promising strategy for constructing MXene or MOF-based aptasensors with high sensitivity, selectivity, and stability for practical hormone monitoring applications.</p> Graphical Abstract <p></p>

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An electrochemical aptasensor for hydrocortisone detection using V-PMOF-derived porous nanomaterials from V2CTx precursors

  • 期刊论文

  • 发表日期：

    2025年9月11日

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  • Zhuo Shi,
  • Shuyi Yang,
  • Zhenxia Ma,
  • Zifeng Wang,
  • Zhanhong Li,
  • Linlin Wang,
  • Ľubomír Švorc,
  • Zhigang Zhu

摘要

An electrochemical aptasensor for hydrocortisone (HC) detection was developed using a vanadium-based porphyrinic metal–organic framework (V-PMOF) materials synthesized from vanadium-based MXene (V₂CTₓ) and tetrakis (4-carboxyphenyl) porphyrin (TCPP), followed by thermal treatment at 800 °C to obtain V-PMOF800°C. The resulting V-PMOF800°C retained a nanoflower-like porous structure, enhancing electron transport, structural integrity, and electroactive site accessibility. Gold nanoparticles (Au NPs) were electrodeposited onto the V-PMOF800°C-modified electrode to improve conductivity and enable thiolated DNA aptamer (DNAapt) immobilization via Au–S bonding. Electrochemical measurements, particularly differential pulse voltammetry (DPV), were employed to evaluate the performance of the aptasensor, which exhibited a wide linear concentration range (0.05 nM–5 μM) and a low detection limit (LOD) of 10 pM. This work introduces a promising strategy for constructing MXene or MOF-based aptasensors with high sensitivity, selectivity, and stability for practical hormone monitoring applications.

Graphical Abstract