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<p>Ofloxacin (OFL) overuse leads to hazardous residues in food and the environment, necessitating sensitive and accurate detection. Traditional gold nanoparticle (AuNP)-based lateral flow immunoassays (LFIAs) suffer from low sensitivity, compromised accuracy, and reliance on single-mode signals, limiting their reliability. We synthesized Au@PdPt nanoparticles (NPs) via a one-step reduction method, integrating a plasmonic Au core with a Pd–Pt alloy shell to enable dual-mode colorimetric and photothermal detection of OFL&#xa0;with enhanced precision. The NPs exhibited broad-spectrum absorption, high photothermal efficiency, and stability. Based on this material, a dual-mode LFIA was developed for OFL, achieving limits of detection (LOD) of 0.059&#xa0;ng&#xa0;mL⁻<sup>1</sup> (colorimetric mode) and 0.038&#xa0;ng&#xa0;mL⁻<sup>1</sup> (photothermal mode), surpassing AuNPs-LFIA by 1.64- and 2.55-fold, respectively. Recovery test in river water and milk samples ranged from 80.72 to 115.24% with relative standard deviations (RSD) below 12.75%, demonstrating high accuracy and reliability in complex matrices. This study offers a reliable, user-friendly platform for OFL detection with potential applications in food safety and environmental monitoring.</p> Graphical Abstract <p></p>

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Au@PdPt nanoparticles-based colorimetric and photothermal dual-mode lateral flow immunoassay for the sensitive detection of ofloxacin

  • 期刊论文

  • 发表日期：

    2025年7月15日

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  • Jialin Zhang,
  • Li Nan,
  • Liang Luo,
  • Zizhe Li,
  • Shumei Zheng,
  • Xuefeng Xia,
  • Chen Xing,
  • Zhanhui Wang,
  • Yantong Pan,
  • Kai Wen

摘要

Ofloxacin (OFL) overuse leads to hazardous residues in food and the environment, necessitating sensitive and accurate detection. Traditional gold nanoparticle (AuNP)-based lateral flow immunoassays (LFIAs) suffer from low sensitivity, compromised accuracy, and reliance on single-mode signals, limiting their reliability. We synthesized Au@PdPt nanoparticles (NPs) via a one-step reduction method, integrating a plasmonic Au core with a Pd–Pt alloy shell to enable dual-mode colorimetric and photothermal detection of OFL with enhanced precision. The NPs exhibited broad-spectrum absorption, high photothermal efficiency, and stability. Based on this material, a dual-mode LFIA was developed for OFL, achieving limits of detection (LOD) of 0.059 ng mL⁻1 (colorimetric mode) and 0.038 ng mL⁻1 (photothermal mode), surpassing AuNPs-LFIA by 1.64- and 2.55-fold, respectively. Recovery test in river water and milk samples ranged from 80.72 to 115.24% with relative standard deviations (RSD) below 12.75%, demonstrating high accuracy and reliability in complex matrices. This study offers a reliable, user-friendly platform for OFL detection with potential applications in food safety and environmental monitoring.

Graphical Abstract