<p><i>Staphylococcus aureus</i>, a prevalent foodborne pathogenic bacterium, poses a significant threat to human health. Herein, a dual-mode sensing method is&#xa0;proposed for detecting <i>S. aureus</i> in food using aptamer-modified Fe/Zr metal organic framework (Apt@Fe/Zr MOF). The Apt@Fe/Zr MOF was prepared through self-assembly of Fe/Zr MOF followed by surface modification with an aptamer. It exhibits peroxidase-like activity, catalyzing the&#xa0;3,3′,5,5′-tetramethylbenzidine (TMB) chromogenic reaction. Meanwhile, Apt@Fe/Zr MOF demonstrates fluorescent characteristics, emitting a fluorescence signal at 428&#xa0;nm upon excitation with light at 350&#xa0;nm. Based on this feature, dual-mode sensing of <i>S. aureus</i> with comparable specificity and excellent anti-interference was achieved through the integration of colorimetry and fluorometry. The limits of detection (LOD) for both colorimetric and fluorometric analyses were 3.25 × 10¹ and 1.47 × 10¹&#xa0;CFU&#xa0;mL⁻¹, respectively. Actual detection of food samples indicates that this method exhibits excellent reliability and accuracy, with recoveries ranging from 91.12 to 107.55% (colorimetry) and 94.40 to 109.47% (fluorometry). The results are highly consistent with those obtained from traditional culture-based methods. Therefore, it is evident that the dual-mode detection of <i>S. aureus</i> using Apt@Fe/Zr MOF offers a rapid and reliable method for ensuring food safety.</p> Graphical Abstract <p></p>