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<p>A&#xa0;dual-mode fluorescent/colorimetric sensor is presented&#xa0;utilizing Au/ZIF-67 nanocomposites for an ultrasensitive ochratoxin A (OTA) detection. The system employs polydopamine-coated Fe<sub>3</sub>O<sub>4</sub> nanoparticles (Fe<sub>3</sub>O<sub>4</sub>@MIP) as magnetic recognition elements and aptamer/3,3′,5,5′-tetramethylbenzidine (TMB)-functionalized Au/ZIF-67 nanocomposites (Apt/TMB/Au/ZIF-67) as dual-signal probes for both fluorescence and colorimetric detection. Through molecular imprinting-aptamer synergistic recognition, OTA binding induces the formation of sandwich-structured particles (Fe<sub>3</sub>O<sub>4</sub>@MIP-OTA-Apt/TMB/Au/ZIF-67). Magnetic separation of these particles facilitates fluorescence measurements, while residual Apt/TMB/Au/ZIF-67 in solution permits simultaneous colorimetric detection. Crucially, fluorescence intensity increases while colorimetric absorption decreases proportionally with OTA concentration (0.5–25&#xa0;ng&#xa0;mL⁻<sup>1</sup>), enabling self-validating dual-mode detection without requiring reagent replacement. The platform achieves detection limits of 0.15&#xa0;ng&#xa0;mL⁻<sup>1</sup> (fluorescence) and 0.13&#xa0;ng&#xa0;mL⁻<sup>1</sup> (colorimetry). The sensor was successfully validated for OTA quantification in real food samples, demonstrating significant potential for food safety applications.</p> Graphical Abstract <p></p>

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Synergistic aptamer/molecularly imprinted polymer-driven fluorescence/colorimetric dual-mode sensor based on Au/ZIF-67 nanocomposites for ultrasensitive ochratoxin A detection

  • 期刊论文

  • 发表日期：

    2025年7月29日

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  • Yufan Yang,
  • Bo Zhao,
  • Xiaopeng Hu,
  • Huiwen Gu,
  • Xiu-Fang Yan,
  • Xiaoli Yin

摘要

A dual-mode fluorescent/colorimetric sensor is presented utilizing Au/ZIF-67 nanocomposites for an ultrasensitive ochratoxin A (OTA) detection. The system employs polydopamine-coated Fe3O4 nanoparticles (Fe3O4@MIP) as magnetic recognition elements and aptamer/3,3′,5,5′-tetramethylbenzidine (TMB)-functionalized Au/ZIF-67 nanocomposites (Apt/TMB/Au/ZIF-67) as dual-signal probes for both fluorescence and colorimetric detection. Through molecular imprinting-aptamer synergistic recognition, OTA binding induces the formation of sandwich-structured particles (Fe3O4@MIP-OTA-Apt/TMB/Au/ZIF-67). Magnetic separation of these particles facilitates fluorescence measurements, while residual Apt/TMB/Au/ZIF-67 in solution permits simultaneous colorimetric detection. Crucially, fluorescence intensity increases while colorimetric absorption decreases proportionally with OTA concentration (0.5–25 ng mL⁻1), enabling self-validating dual-mode detection without requiring reagent replacement. The platform achieves detection limits of 0.15 ng mL⁻1 (fluorescence) and 0.13 ng mL⁻1 (colorimetry). The sensor was successfully validated for OTA quantification in real food samples, demonstrating significant potential for food safety applications.

Graphical Abstract