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<p>Au NPs@FGO@Fe MOG were sythesized&#xa0;for its inaugural application in biosensor development and engineered as a novel, highly efficient electrochemical aptasensor for the precise detection of interleukin-6 (IL-6). The aptasensor architecture integrates Au NPs@FGO@Fe MOG as the electrode interface and an anemone-like PtNi nanoclusters@MIL-anchored three-dimensional DNA (PD) network as the signal transduction matrix. The as-prepared Au NPs@FGO@Fe MOG exhibited an exceptionally high specific surface area, excellent electrical conductivity, and robust structural stability, collectively contributing to a pronounced enhancement in the performance of an&#xa0;electrochemical biosensor. The&#xa0;hierarchically PD network provided abundant binding sites for the redox-active probe thionine (Thi), thereby amplifying the electrochemical signal with remarkable efficiency. Under optimized conditions, the aptasensor achieved an impressive detection limit of 0.89 pg/mL. Moreover, the platform demonstrated reliable performance in detecting IL-6 in unspiked human serum samples, with results exhibiting high concordance with those obtained from commercial enzyme-linked immunosorbent assay (ELISA) kits. These findings demonstrate the considerable potential of this platform for rapid, sensitive, and accurate cytokine quantification, offering valuable prospects for clinical diagnostic applications.</p> Graphical Abstract <p></p>

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A multi-thionine DNA network-driven electrochemical aptasensor with dual signal amplification for ultrasensitive interleukin-6 detection

  • 期刊论文

  • 发表日期：

    2025年9月12日

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  • Linru Bai,
  • Benqi Chen,
  • Yan Xu,
  • Mingjia Liu,
  • Yunhui Yang,
  • Jianmei Yang,
  • Rong Hu

摘要

Au NPs@FGO@Fe MOG were sythesized for its inaugural application in biosensor development and engineered as a novel, highly efficient electrochemical aptasensor for the precise detection of interleukin-6 (IL-6). The aptasensor architecture integrates Au NPs@FGO@Fe MOG as the electrode interface and an anemone-like PtNi nanoclusters@MIL-anchored three-dimensional DNA (PD) network as the signal transduction matrix. The as-prepared Au NPs@FGO@Fe MOG exhibited an exceptionally high specific surface area, excellent electrical conductivity, and robust structural stability, collectively contributing to a pronounced enhancement in the performance of an electrochemical biosensor. The hierarchically PD network provided abundant binding sites for the redox-active probe thionine (Thi), thereby amplifying the electrochemical signal with remarkable efficiency. Under optimized conditions, the aptasensor achieved an impressive detection limit of 0.89 pg/mL. Moreover, the platform demonstrated reliable performance in detecting IL-6 in unspiked human serum samples, with results exhibiting high concordance with those obtained from commercial enzyme-linked immunosorbent assay (ELISA) kits. These findings demonstrate the considerable potential of this platform for rapid, sensitive, and accurate cytokine quantification, offering valuable prospects for clinical diagnostic applications.

Graphical Abstract