Scholarlink

<p>Accurately detecting cell secretions in complex microenvironments is crucial for understanding cellular communication, disease progression, and therapeutic responses. Traditional methods, such as ELISA, provide limited insight into the spatiotemporal dynamics of secretions, often requiring invasive or endpoint analyses. To address these challenges, we have functionalized the CellStudio platform—previously developed to integrate cell adhesion areas and microbeads patterns—with a novel self-reporting structure-switching signaling aptamer (SSSA) for vascular endothelial growth factor (VEGF) detection. This functionalization enables in situ single-step, real-time quantification of VEGF secretion from hundreds of live mesenchymal stem cell (MSC) clusters over a 24-h period, using standard fluorescence microscopy without the need for cell fixation. The spatial resolution provided by this system enables precise, localized monitoring of secretion events without the need to add extra reagents. This user-friendly platform is easily adaptable to conventional laboratory workflows, offering a versatile tool with potential for studying cellular behavior in real time with minimal technical barriers.</p> Graphical abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Non-invasive detection of VEGF secretion from small clusters of mesenchymal stem cells using VEGF-SSSA integrated into the CellStudio platform

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年7月5日

查看全文
  • Enrique Azuaje-Hualde,
  • Naiara Lartitegui-Meneses,
  • Fernando Benito-Lopez,
  • Lourdes Basabe-Desmonts

摘要

Accurately detecting cell secretions in complex microenvironments is crucial for understanding cellular communication, disease progression, and therapeutic responses. Traditional methods, such as ELISA, provide limited insight into the spatiotemporal dynamics of secretions, often requiring invasive or endpoint analyses. To address these challenges, we have functionalized the CellStudio platform—previously developed to integrate cell adhesion areas and microbeads patterns—with a novel self-reporting structure-switching signaling aptamer (SSSA) for vascular endothelial growth factor (VEGF) detection. This functionalization enables in situ single-step, real-time quantification of VEGF secretion from hundreds of live mesenchymal stem cell (MSC) clusters over a 24-h period, using standard fluorescence microscopy without the need for cell fixation. The spatial resolution provided by this system enables precise, localized monitoring of secretion events without the need to add extra reagents. This user-friendly platform is easily adaptable to conventional laboratory workflows, offering a versatile tool with potential for studying cellular behavior in real time with minimal technical barriers.

Graphical abstract