Scholarlink

<p>A novel fluorescent aptasensor was introduced for the assessment of sulfadimethoxine (SDM) using multiple functions of ZrFe metal–organic framework (MOF). This sensing strategy exploits the ZrFe MOF features including the peroxidase-mimicking feature, fluorescence characteristics, single-stranded DNA adsorption on its surface, and the change in its catalytic activity. In the detection mechanism, ferrofluid was used as separation agent and the peroxidized product quenched the fluorescence intensity of the ZrFe MOF through the fluorescence resonance energy transfer (FRET) effect. In this FRET-based approach, the existence or absence of SDM affects the attendance or non-attendance of complementary strand of aptamer (CS) in supernatant solution, which subsequently alters the peroxidase-like behavior of ZrFe MOF. Thus, the discrepancy in fluorescence intensity is caused by the variation in the amount of produced peroxidation product. Employing this sensing platform, a detection limit of 3&#xa0;pM was achieved. Furthermore, the suggested sensing platform, leveraging the specificity of the aptamer for its target, demonstrated a significant potential to mitigate the influence of other interfering substances. The aptasensor has the capability to quantitatively detect SDM content in human serum samples. Therefore, it is well-suited for use in intricate matrices.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

A FRET-based aptasensor exploiting the multiple functions of zirconium-based bimetallic organic frameworks for the quantitative determination of sulfadimethoxine

  • 期刊论文

  • 发表日期：

    2025年5月31日

查看全文
  • Masoomeh Esmaelpourfarkhani,
  • Mahshid Olfati Sumar,
  • Mohammad Ramezani,
  • Mona Alibolandi,
  • Khalil Abnous,
  • Seyed Mohammad Taghdisi

摘要

A novel fluorescent aptasensor was introduced for the assessment of sulfadimethoxine (SDM) using multiple functions of ZrFe metal–organic framework (MOF). This sensing strategy exploits the ZrFe MOF features including the peroxidase-mimicking feature, fluorescence characteristics, single-stranded DNA adsorption on its surface, and the change in its catalytic activity. In the detection mechanism, ferrofluid was used as separation agent and the peroxidized product quenched the fluorescence intensity of the ZrFe MOF through the fluorescence resonance energy transfer (FRET) effect. In this FRET-based approach, the existence or absence of SDM affects the attendance or non-attendance of complementary strand of aptamer (CS) in supernatant solution, which subsequently alters the peroxidase-like behavior of ZrFe MOF. Thus, the discrepancy in fluorescence intensity is caused by the variation in the amount of produced peroxidation product. Employing this sensing platform, a detection limit of 3 pM was achieved. Furthermore, the suggested sensing platform, leveraging the specificity of the aptamer for its target, demonstrated a significant potential to mitigate the influence of other interfering substances. The aptasensor has the capability to quantitatively detect SDM content in human serum samples. Therefore, it is well-suited for use in intricate matrices.

Graphical Abstract