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<p>A ratio fluorescence/colorimetry/smartphone triple-mode sensor based on Ru-C<sub>3</sub>N<sub>4</sub> was established for precise and rapid detection of melatonin (MT). Specifically, Ru-C<sub>3</sub>N<sub>4</sub> with fine luminous characteristic and peroxidase-like activity catalyzes the&#xa0;oxidation of OPD to generate luminescent DAP which in turn suppresses intrinsic fluorescence of Ru-C<sub>3</sub>N<sub>4</sub> because of the&#xa0;fluorescence inner filter effect (IFE). MT can inhibit this catalytic process, leading to partial recovery of the fluorescence of&#xa0;Ru-C<sub>3</sub>N<sub>4</sub> and the decrease in UV–vis signal of the&#xa0;reaction system, as well as the gradual fading of the yellow color&#xa0;of the&#xa0;reaction solution. Hence, this triple-mode sensor can realize precise, reliable, and high-throughput determination of MT with correlative detection limits of 0.32, 0.46, and 0.67&#xa0;μM. Moreover, this triple-mode sensor has been applied to MT detection in multiple juice samples with recoveries ranging from 86.00 to 99.68%, which indicates the suitability of the novel idea for the research and development of multimode optical sensors for food analysis.</p> Graphical Abstract <p></p>

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A ratio fluorescence/colorimetry/smartphone triple-mode sensor based on solvent-dominated signal amplification strategy for precise and rapid detection of melatonin in fruit juice

  • 期刊论文

  • 发表日期：

    2025年5月28日

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  • Linjie Wang,
  • Lu Wang,
  • Lin Jing,
  • Yang Yang,
  • Hui Miao,
  • Xiaoya Liu,
  • Dongzhi Suonanmu,
  • Qingjia Ren,
  • Fei Wang,
  • Caolong Li

摘要

A ratio fluorescence/colorimetry/smartphone triple-mode sensor based on Ru-C3N4 was established for precise and rapid detection of melatonin (MT). Specifically, Ru-C3N4 with fine luminous characteristic and peroxidase-like activity catalyzes the oxidation of OPD to generate luminescent DAP which in turn suppresses intrinsic fluorescence of Ru-C3N4 because of the fluorescence inner filter effect (IFE). MT can inhibit this catalytic process, leading to partial recovery of the fluorescence of Ru-C3N4 and the decrease in UV–vis signal of the reaction system, as well as the gradual fading of the yellow color of the reaction solution. Hence, this triple-mode sensor can realize precise, reliable, and high-throughput determination of MT with correlative detection limits of 0.32, 0.46, and 0.67 μM. Moreover, this triple-mode sensor has been applied to MT detection in multiple juice samples with recoveries ranging from 86.00 to 99.68%, which indicates the suitability of the novel idea for the research and development of multimode optical sensors for food analysis.

Graphical Abstract