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<p>Alterations in glycosylation patterns have been observed in many diseases, including inflammatory, auto-immune, and infectious diseases as well as cancer. However, the low concentrations of glycoproteins in biological samples make it difficult to enrich and separate glycoproteins efficiently before determination. In this study, a composite material was prepared&#xa0;in which Fe<sub>3</sub>O<sub>4</sub>-based magnetic nanospheres were modified with polyethyleneimine (PEI)- and boric acid-grafted metal–organic frameworks (MOFs) to enable the selective enrichment and separation of glycoproteins from complex biological samples. With the coordination between amino and boric acid, a&#xa0;teamed boronate affinity (TBA) molecular group is formed, which enables the cyclization of boric acid and the <i>cis</i>-diol groups in glycoproteins to be achieved at physiological pH. In addition, the abundant hydrogen bonds between amino and glycoprotein also increase the affinity of the synthesized Fe<sub>3</sub>O<sub>4</sub>@MOF@PEI@BBA nanoparticles for glycoprotein. The adsorption capacities for ovalbumin, transferrin, and horseradish peroxidase were 489.3, 522.0, and 578.2&#xa0;mg/g, respectively. Meanwhile, adsorption equilibrium was achieved within 30&#xa0;min under mild physiological conditions (pH = 7.4, T = 25&#xa0;°C). </p> Graphical Abstract <p></p>

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A simple and highly efficient method for enriching and separating glycoproteins in complex biological samples using polyethyleneimine- and boric acid-grafted metal–organic frameworks on magnetic nanospheres

  • 期刊论文

  • 发表日期：

    2025年6月23日

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  • Hang Gong,
  • Ruirui Hu,
  • Feng Chen,
  • Yong Li,
  • Tao Pang,
  • Chunyan Chen,
  • Changqun Cai

摘要

Alterations in glycosylation patterns have been observed in many diseases, including inflammatory, auto-immune, and infectious diseases as well as cancer. However, the low concentrations of glycoproteins in biological samples make it difficult to enrich and separate glycoproteins efficiently before determination. In this study, a composite material was prepared in which Fe3O4-based magnetic nanospheres were modified with polyethyleneimine (PEI)- and boric acid-grafted metal–organic frameworks (MOFs) to enable the selective enrichment and separation of glycoproteins from complex biological samples. With the coordination between amino and boric acid, a teamed boronate affinity (TBA) molecular group is formed, which enables the cyclization of boric acid and the cis-diol groups in glycoproteins to be achieved at physiological pH. In addition, the abundant hydrogen bonds between amino and glycoprotein also increase the affinity of the synthesized Fe3O4@MOF@PEI@BBA nanoparticles for glycoprotein. The adsorption capacities for ovalbumin, transferrin, and horseradish peroxidase were 489.3, 522.0, and 578.2 mg/g, respectively. Meanwhile, adsorption equilibrium was achieved within 30 min under mild physiological conditions (pH = 7.4, T = 25 °C).

Graphical Abstract