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<p> Neuraminidase (NA), a highly sensitive recognition element for anti-influenza activity, was immobilized on a glassy carbon electrode modified with nitrogen-doped graphene-functionalized multi-walled carbon nanotube composites (N-Gr/MWCNTs) and polypyrrole (Ppy) via re-polymerization. A highly sensitive electrochemical biosensor was thus&#xa0;developed for the rapid screening of antiviral components in natural products, utilizing the high specific surface area and conductivity of N-Gr/MWCNTs and polypyrrole, along with the high-throughput and specific recognition capabilities of NA. Under optimized conditions, the sensor was used to assess five distinct classes of compounds, demonstrating an activity sequence of Baicalein &gt; Baicalin &gt; Rutin ≈ Matrine &gt; Levistilide A, which aligns with previous literature. This study presents a convenient and efficient method for screening antiviral components in natural products, providing new strategies and technological support for the development of cost-effective, eco-friendly, and rapid screening platforms.</p> Graphical Abstract <p></p>

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A neuraminidase-based electrochemical biosensor for high-throughput screening of antiviral compounds in traditional Chinese medicine

  • 期刊论文

  • 发表日期：

    2025年5月27日

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  • Miao Zhou,
  • Bolu Sun,
  • Yuqiong Wu,
  • Huajie Li,
  • Hai Wang,
  • Xuanxiu Da,
  • Ying Lv,
  • Xinlan Wang,
  • Xiuying Pu,
  • Lin Yang,
  • Ke Sun,
  • Guolin Chai

摘要

Neuraminidase (NA), a highly sensitive recognition element for anti-influenza activity, was immobilized on a glassy carbon electrode modified with nitrogen-doped graphene-functionalized multi-walled carbon nanotube composites (N-Gr/MWCNTs) and polypyrrole (Ppy) via re-polymerization. A highly sensitive electrochemical biosensor was thus developed for the rapid screening of antiviral components in natural products, utilizing the high specific surface area and conductivity of N-Gr/MWCNTs and polypyrrole, along with the high-throughput and specific recognition capabilities of NA. Under optimized conditions, the sensor was used to assess five distinct classes of compounds, demonstrating an activity sequence of Baicalein > Baicalin > Rutin ≈ Matrine > Levistilide A, which aligns with previous literature. This study presents a convenient and efficient method for screening antiviral components in natural products, providing new strategies and technological support for the development of cost-effective, eco-friendly, and rapid screening platforms.

Graphical Abstract