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<p>In the field of biosensing and chemical sensing, there is a growing demand for multiplexed detection and quantification of multiple targets within complex matrices. In electrochemical sensing, simultaneous multiplexed analysis is typically performed with multiple electrodes connected to a multichannel potentiostat. An alternative strategy involves using a single electrode capable of discriminating and detecting several analytes in a single measurement, which is, however,&#xa0;unfortunately limited to a selective group of molecules. Herein, we report a novel electrochemical method based on the parallel&#xa0;assembly of a&#xa0;dual-electrochemical cell (PADEC), which enables the simultaneous detection and quantification of solvent-incompatible analytes, prepared separately in two distinct electrochemical cells, using a single-channel potentiostat—thus achieving multichannel-like performance. This approach relies on connecting two electrochemical cells in parallel, allowing the concurrent measurement of distinct electrochemical responses from analytes that otherwise could not be simultaneously determined&#xa0; due to solvent incompatibility. As a proof of concept, the water-soluble vitamin C, and the lipid-soluble vitamin D3 were simultaneously determined, each in its respective optimized medium. The PADEC approach demonstrated performance comparable to individual detection methods, achieving limits of detection of 27 μM for vitamin C and 32 μM for vitamin D3 over a linear range of 20–400 μM. This strategy establishes a new approach for simultaneous, multiplexed electrochemical determination&#xa0; of analytes in different media. Moreover, this innovation may extend applications in electrochemistry beyond (bio)sensing to include areas such as electrocatalysis, energy and corrosion, potentially reducing dependence on multichannel potentiostats.</p> Graphical Abstract <p></p>

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Parallel assembly of dual-electrochemical cell: a novel approach for simultaneous multiplexed sensing analysis

  • 期刊论文

  • 发表日期：

    2025年5月7日

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  • Pedro V. V. Romanholo,
  • Larissa M. Andrade,
  • Marcelo Giglioti,
  • Guilherme Z. A. Luccas,
  • Sergio A. S. Machado,
  • Livia F. Sgobbi

摘要

In the field of biosensing and chemical sensing, there is a growing demand for multiplexed detection and quantification of multiple targets within complex matrices. In electrochemical sensing, simultaneous multiplexed analysis is typically performed with multiple electrodes connected to a multichannel potentiostat. An alternative strategy involves using a single electrode capable of discriminating and detecting several analytes in a single measurement, which is, however, unfortunately limited to a selective group of molecules. Herein, we report a novel electrochemical method based on the parallel assembly of a dual-electrochemical cell (PADEC), which enables the simultaneous detection and quantification of solvent-incompatible analytes, prepared separately in two distinct electrochemical cells, using a single-channel potentiostat—thus achieving multichannel-like performance. This approach relies on connecting two electrochemical cells in parallel, allowing the concurrent measurement of distinct electrochemical responses from analytes that otherwise could not be simultaneously determined  due to solvent incompatibility. As a proof of concept, the water-soluble vitamin C, and the lipid-soluble vitamin D3 were simultaneously determined, each in its respective optimized medium. The PADEC approach demonstrated performance comparable to individual detection methods, achieving limits of detection of 27 μM for vitamin C and 32 μM for vitamin D3 over a linear range of 20–400 μM. This strategy establishes a new approach for simultaneous, multiplexed electrochemical determination  of analytes in different media. Moreover, this innovation may extend applications in electrochemistry beyond (bio)sensing to include areas such as electrocatalysis, energy and corrosion, potentially reducing dependence on multichannel potentiostats.

Graphical Abstract