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<p> A&#xa0;novel cerium phthalocyanine-based covalent organic framework (CePc-COF)-magnetic core–shell composite was fabricated by grafting the CePc-COF layer on the surface of Fe<sub>3</sub>O<sub>4</sub>. The obtained core–shell composite particle (Fe<sub>3</sub>O<sub>4</sub>@CePc-COF) had strong magnetic responsiveness (29.6 emu g<sup>−1</sup>) and good hydrophilicity (5.0°). The affinity material provided abundant metal ion sites for specific enrichment of phosphopeptides. Fe<sub>3</sub>O<sub>4</sub>@CePc-COF had good performance in terms of high selectivity (1: 1: 5000), sensitivity (0.1 fmol), recovery (92.91%), and reusability (10 cycles). The enrichment feasibility of Fe<sub>3</sub>O<sub>4</sub>@CePc-COF was first investigated in standard peptides. Furthermore, Fe<sub>3</sub>O<sub>4</sub>@CePc-COF can efficiently identify phosphopeptides from extremely complex samples. The work can provide a novel idea for the fabrication of metallophthalocyanine based-COF materials for phosproteome detection in biological samples.</p> Graphical Abstract <p></p>

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A cerium phthalocyanine-based covalent organic framework-magnetic core–shell composite as efficient affinity material for the enrichment of phosphopeptides

  • 期刊论文

  • 发表日期：

    2025年3月25日

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  • Dandan Jiang,
  • Yangyang Li,
  • Siyu Wu,
  • Lan Lan,
  • Jinghai Liu

摘要

A novel cerium phthalocyanine-based covalent organic framework (CePc-COF)-magnetic core–shell composite was fabricated by grafting the CePc-COF layer on the surface of Fe3O4. The obtained core–shell composite particle (Fe3O4@CePc-COF) had strong magnetic responsiveness (29.6 emu g−1) and good hydrophilicity (5.0°). The affinity material provided abundant metal ion sites for specific enrichment of phosphopeptides. Fe3O4@CePc-COF had good performance in terms of high selectivity (1: 1: 5000), sensitivity (0.1 fmol), recovery (92.91%), and reusability (10 cycles). The enrichment feasibility of Fe3O4@CePc-COF was first investigated in standard peptides. Furthermore, Fe3O4@CePc-COF can efficiently identify phosphopeptides from extremely complex samples. The work can provide a novel idea for the fabrication of metallophthalocyanine based-COF materials for phosproteome detection in biological samples.

Graphical Abstract