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<p> A&#xa0;novel electrochemical biosensor for the detection of lead ions (Pb<sup>2+</sup>) with improved specificity and sensitivity&#xa0;was developed. The sensor design incorporated molecular imprinting technology, where chitosan was polymerized on the electrode surface to form a lead-specific cavity structure, thereby enhancing selectivity in complex sample matrices. Meanwhile, the tetrahedral DNA nanostructure was employed as the recognition probe to mitigate the entanglement issues commonly associated with single-stranded DNA, thus improving the sensitivity of the detection. The developed sensor exhibited a linear dynamic range from 0.050 to 2.000&#xa0;μg/mL, with a limit of detection (LOD) of 0.0034&#xa0;μg/mL. The aptasensor’s efficacy was verified through the analysis of aquatic samples, demonstrating a high degree of reliability comparable to that of inductively coupled plasma mass spectrometry (ICP-MS).</p> Graphical Abstract <p></p>

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A highly selective electrochemical aptasensor for Pb2+ based on molecular imprinting technology and tetrahedral DNA nanostructure

  • 期刊论文

  • 发表日期：

    2025年4月2日

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  • Jiaqi Han,
  • Nianxin Zhu,
  • Jiahao Sha,
  • Jinyan Cai,
  • Hui Cao,
  • Tai Ye,
  • Liling Hao,
  • Fei Xu

摘要

A novel electrochemical biosensor for the detection of lead ions (Pb2+) with improved specificity and sensitivity was developed. The sensor design incorporated molecular imprinting technology, where chitosan was polymerized on the electrode surface to form a lead-specific cavity structure, thereby enhancing selectivity in complex sample matrices. Meanwhile, the tetrahedral DNA nanostructure was employed as the recognition probe to mitigate the entanglement issues commonly associated with single-stranded DNA, thus improving the sensitivity of the detection. The developed sensor exhibited a linear dynamic range from 0.050 to 2.000 μg/mL, with a limit of detection (LOD) of 0.0034 μg/mL. The aptasensor’s efficacy was verified through the analysis of aquatic samples, demonstrating a high degree of reliability comparable to that of inductively coupled plasma mass spectrometry (ICP-MS).

Graphical Abstract