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<p> Nanozyme-linked immunosorbent assay has emerged as a promising strategy for sensitive biosensing. However, the catalytic activity and stability of nanozymes affect the accuracy of immunosorbent assays. In this study, we synthesized a Pt/DMSN nanozyme with peroxidase-mimicking activity, which effectively catalyzed the oxidation of peroxidase substrate 3,3',5,5'-tetramethylbenzidin&#xa0;(TMB) in the presence of hydrogen peroxide. Capitalizing on its peroxidase-like activity, the Pt/DMSN nanozyme was functionalized with dual-fluorescent recognition elements (HER2-mAbs and sk6Ea aptamers) to establish a nanozyme-linked immunosorbent assay platform, which exhibited catalytic stability and substrate affinity comparable to horseradish peroxidase. The resulting Multi-Pt/DMSN platform was used to selectively distinguish HER2-positive breast cancer cells from luminal A, triple-negative breast cancer subtypes, and non-neoplastic cells, achieving a detection limit of 50 HER2-positive cells within 30&#xa0;min. The combination of robust enzyme-like activity and tumor-targeting properties enables fluorescence imaging, providing dual-mode diagnostic functionality. This work presents a prospective platform for differentiating breast cancer subtypes in early diagnosis.</p> Graphical abstract <p></p>

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A multifunctional Pt/DMSN nanozyme-based colorimetric-fluorescence sensing platform for breast cancer detection

  • 期刊论文

  • 发表日期：

    2025年3月13日

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  • Xinrui Xue,
  • Fang Zheng,
  • Yujia Luo,
  • Wenyu Chen,
  • Yuanyuan Gao,
  • Kun Wei

摘要

Nanozyme-linked immunosorbent assay has emerged as a promising strategy for sensitive biosensing. However, the catalytic activity and stability of nanozymes affect the accuracy of immunosorbent assays. In this study, we synthesized a Pt/DMSN nanozyme with peroxidase-mimicking activity, which effectively catalyzed the oxidation of peroxidase substrate 3,3',5,5'-tetramethylbenzidin (TMB) in the presence of hydrogen peroxide. Capitalizing on its peroxidase-like activity, the Pt/DMSN nanozyme was functionalized with dual-fluorescent recognition elements (HER2-mAbs and sk6Ea aptamers) to establish a nanozyme-linked immunosorbent assay platform, which exhibited catalytic stability and substrate affinity comparable to horseradish peroxidase. The resulting Multi-Pt/DMSN platform was used to selectively distinguish HER2-positive breast cancer cells from luminal A, triple-negative breast cancer subtypes, and non-neoplastic cells, achieving a detection limit of 50 HER2-positive cells within 30 min. The combination of robust enzyme-like activity and tumor-targeting properties enables fluorescence imaging, providing dual-mode diagnostic functionality. This work presents a prospective platform for differentiating breast cancer subtypes in early diagnosis.

Graphical abstract