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<p>IL-17 (interleukin-17) is a common proinflammatory cytokine and can be adopted as an important biomarker for several diseases; it is thus very necessary to design an effective method for monitoring IL-17. Herein, by preparing silver nanoparticles/graphdiyne (Ag/GDY) with simple electroless deposition as the sensing platform and methylene blue/UiO-66-NH<sub>2</sub> MOFs (MB/MOFs) as the signaling-amplifier, a highly efficient sandwich-like electrochemical immunosensing (SLEI) platform was designed for IL-17 detection. The superior electrical property, easy preparation, large surface area, and high stability of Ag/GDY make it a desirable substrate to immobilize primary antibody of IL-17. Meanwhile, the unique structure of UiO-66-NH<sub>2</sub> is beneficial to immobilize MB probe and secondary antibody. By optimizing experimental conditions, the MB current obtained at the as-designed SLEI platform increases along with IL-17 increase, and the related linearity is 1.0&#xa0;pg&#xa0;mL<sup>−1</sup>–1.0&#xa0;ng&#xa0;mL<sup>−1</sup> coupled with a low detection limit of 0.3&#xa0;pg&#xa0;mL<sup>−1</sup>. It is expected that the SLEI platform based on Ag/GDY and MB/MOFs shows some potential application for IL-17 detection.</p> Graphical abstract <p></p>

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Sandwich-like electrochemical immunosensing of IL-17 based on silver nanoparticles/graphdiyne and methylene blue/UiO-66-NH2

  • 期刊论文

  • 发表日期：

    2025年4月17日

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  • Rui Li,
  • Wenzhong Que,
  • Haiyan Han,
  • Honghui Shi,
  • Rongfeng Wang,
  • Bin Qiu

摘要

IL-17 (interleukin-17) is a common proinflammatory cytokine and can be adopted as an important biomarker for several diseases; it is thus very necessary to design an effective method for monitoring IL-17. Herein, by preparing silver nanoparticles/graphdiyne (Ag/GDY) with simple electroless deposition as the sensing platform and methylene blue/UiO-66-NH2 MOFs (MB/MOFs) as the signaling-amplifier, a highly efficient sandwich-like electrochemical immunosensing (SLEI) platform was designed for IL-17 detection. The superior electrical property, easy preparation, large surface area, and high stability of Ag/GDY make it a desirable substrate to immobilize primary antibody of IL-17. Meanwhile, the unique structure of UiO-66-NH2 is beneficial to immobilize MB probe and secondary antibody. By optimizing experimental conditions, the MB current obtained at the as-designed SLEI platform increases along with IL-17 increase, and the related linearity is 1.0 pg mL−1–1.0 ng mL−1 coupled with a low detection limit of 0.3 pg mL−1. It is expected that the SLEI platform based on Ag/GDY and MB/MOFs shows some potential application for IL-17 detection.

Graphical abstract