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<p>Bimetallic Cu<sub>0.5</sub>Ru<sub>0.5</sub> nanozymes were synthesized by alloying Ru with inexpensive Cu through a polyol method for hypoxanthine sensing. The prepared Cu<sub>0.5</sub>Ru<sub>0.5</sub> nanozymes exhibit a peroxidase-like activity. Under the specific enzyme of xanthine oxidase (XOD), hypoxanthine was first converted to xanthine, and then to the final product uric acid, accompanied by the production of H<sub>2</sub>O<sub>2</sub>. Cu<sub>0.5</sub>Ru<sub>0.5</sub> nanozymes could catalyze H<sub>2</sub>O<sub>2</sub> to generate •OH and then oxidize colorless TMB to blue oxTMB, resulting in a&#xa0;visible color change. Therefore, the determination of hypoxanthine was achieved by monitoring the absorption value change of oxTMB at 652 nm. The proposed hypoxanthine sensing strategy displayed a high sensitivity and selectivity, with the limit of detection of 0.5 μM. Furthermore, we designed a rapid and convenient platform for hypoxanthine detection using a smartphone with image recognition and data processing function.</p> Graphical abstract <p></p>

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Bimetallic CuRu nanozymes for colorimetric and smartphone-assisted rapid visual hypoxanthine biosensing in serum samples

  • 期刊论文

  • 发表日期：

    2025年2月13日

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  • Aifang Zhou,
  • Juan Chen,
  • Xintong Dong,
  • Pin Bai,
  • Qianqian Zhu,
  • Hong-Min Meng,
  • Zhaohui Li

摘要

Bimetallic Cu0.5Ru0.5 nanozymes were synthesized by alloying Ru with inexpensive Cu through a polyol method for hypoxanthine sensing. The prepared Cu0.5Ru0.5 nanozymes exhibit a peroxidase-like activity. Under the specific enzyme of xanthine oxidase (XOD), hypoxanthine was first converted to xanthine, and then to the final product uric acid, accompanied by the production of H2O2. Cu0.5Ru0.5 nanozymes could catalyze H2O2 to generate •OH and then oxidize colorless TMB to blue oxTMB, resulting in a visible color change. Therefore, the determination of hypoxanthine was achieved by monitoring the absorption value change of oxTMB at 652 nm. The proposed hypoxanthine sensing strategy displayed a high sensitivity and selectivity, with the limit of detection of 0.5 μM. Furthermore, we designed a rapid and convenient platform for hypoxanthine detection using a smartphone with image recognition and data processing function.

Graphical abstract