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<p>Penicillin, a widely used narrow-spectrum antibiotic, remains the most frequently reported drug allergy. However, less than 20% of individuals reporting a penicillin allergy are truly allergic. Antibiotic allergy de-labeling is, therefore, essential to prevent adverse reactions and inappropriate treatments. We present the first single-step Lateral Flow Immunoassay for penicillin G allergy screening, offering real-time visual results at the point of care. The detection limit of 0.3&#xa0;IU/mL of the assay, equivalent to 0.72&#xa0;µg/L, determined in artificial human serum, enables the identification of class 0 allergy patients (&lt; 0.35&#xa0;IU/mL) per the RAST scale, distinguishing between low, medium, and high allergy severity groups with a smartphone reader over a dynamic range of 0.3–10&#xa0;IU/mL. This lateral flow assay for penicillin allergy screening enables a shift in allergy diagnostics from centralized laboratories to more accessible point-of-care settings, including doctors’ offices and self-testing.</p> Graphical Abstract <p></p>

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Development of a single-step lateral flow immunoassay for penicillin G allergy screening

  • 期刊论文

  • 发表日期：

    2025年1月31日

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  • Pedro Quintero-Campos,
  • Yulieth D. Banguera-Ordoñez,
  • Amadeo Sena-Torralba,
  • Ángel Maquieira,
  • Sergi Morais

摘要

Penicillin, a widely used narrow-spectrum antibiotic, remains the most frequently reported drug allergy. However, less than 20% of individuals reporting a penicillin allergy are truly allergic. Antibiotic allergy de-labeling is, therefore, essential to prevent adverse reactions and inappropriate treatments. We present the first single-step Lateral Flow Immunoassay for penicillin G allergy screening, offering real-time visual results at the point of care. The detection limit of 0.3 IU/mL of the assay, equivalent to 0.72 µg/L, determined in artificial human serum, enables the identification of class 0 allergy patients (< 0.35 IU/mL) per the RAST scale, distinguishing between low, medium, and high allergy severity groups with a smartphone reader over a dynamic range of 0.3–10 IU/mL. This lateral flow assay for penicillin allergy screening enables a shift in allergy diagnostics from centralized laboratories to more accessible point-of-care settings, including doctors’ offices and self-testing.

Graphical Abstract