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<p> An&#xa0;aptasensor was developed&#xa0;based on surface-enhanced Raman spectroscopy (SERS) to detect alpha-fetoprotein (AFP) using a mirror aptamer. AFP triggered and altered SERS signaling molecules through the release recognition mechanism as they are released. Two signaling molecules, cyanine 3 (Cy3) and 2-mercaptobenzimidazole-5-carboxylic acid (MBIA), were used to quantify AFP. The results showed that AFP could be reliably and sensitively detected in the concentration range 0.0038–100&#xa0;ng/mL (<i>R</i><sup>2</sup> = 0.9907), with a minimum detection limit of 3.8&#xa0;pg/mL. The sensor can be used for AFP detection&#xa0;in cancer patient serum samples. This quantitative SERS technology provides a promising tool for biomedical research and clinical diagnosis.</p> Graphical abstract <p></p>

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Highly stable SERS-based mirrored aptasensor for selective detection of alpha-fetoprotein

  • 期刊论文

  • 发表日期：

    2025年1月31日

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  • Xuning Kang,
  • Zhongmei Peng,
  • Qiulan Li,
  • Zhenlin Fan,
  • Jingping Zhang,
  • Yuzhu Song,
  • Jinyang Zhang,
  • Qinqin Han

摘要

An aptasensor was developed based on surface-enhanced Raman spectroscopy (SERS) to detect alpha-fetoprotein (AFP) using a mirror aptamer. AFP triggered and altered SERS signaling molecules through the release recognition mechanism as they are released. Two signaling molecules, cyanine 3 (Cy3) and 2-mercaptobenzimidazole-5-carboxylic acid (MBIA), were used to quantify AFP. The results showed that AFP could be reliably and sensitively detected in the concentration range 0.0038–100 ng/mL (R2 = 0.9907), with a minimum detection limit of 3.8 pg/mL. The sensor can be used for AFP detection in cancer patient serum samples. This quantitative SERS technology provides a promising tool for biomedical research and clinical diagnosis.

Graphical abstract