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<p><i>Mycoplasma pneumoniae</i> (MP) is a major a rapid, sensitive and reliable method for the detection of 16S ribosomal RNA (16S rRNA) was developed based on catalytic hairpin assembly (CHA) technology and digoxine-biotin double-labeled fluorescence immunochromatography (FICA) strip. The signal amplification achieved by CHA avoids the dependence on expensive thermal cycling equipment. The method can obtain the results within 1&#xa0;h in the absence of an enzyme, and the minimum detection limit can reach 1&#xa0;pM. It has good specificity and can effectively distinguish target sequences from non-target sequences. After verification with adult samples and pediatric samples, it was found that CHA-FICA detection was highly consistent with those of PCR detection, indicating that it has clinical application potential and is expected to be used in clinical screening and treatment detection of <i>mycoplasma pneumoniae </i>(MP) infection.</p> Graphical Abstract <p></p>

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Rapid and facile detection of Mycoplasma pneumoniae 16S rRNA based on CHA-FICA system

  • 期刊论文

  • 发表日期：

    2025年2月1日

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  • Shijie Cai,
  • Yuming Yao,
  • Yaya Chen,
  • Gulinaizhaer Abudushalamua,
  • Shuo Ma,
  • Jiwei Wang,
  • Chen Zhang,
  • Xun Gao,
  • Guoqiu Wu

摘要

Mycoplasma pneumoniae (MP) is a major a rapid, sensitive and reliable method for the detection of 16S ribosomal RNA (16S rRNA) was developed based on catalytic hairpin assembly (CHA) technology and digoxine-biotin double-labeled fluorescence immunochromatography (FICA) strip. The signal amplification achieved by CHA avoids the dependence on expensive thermal cycling equipment. The method can obtain the results within 1 h in the absence of an enzyme, and the minimum detection limit can reach 1 pM. It has good specificity and can effectively distinguish target sequences from non-target sequences. After verification with adult samples and pediatric samples, it was found that CHA-FICA detection was highly consistent with those of PCR detection, indicating that it has clinical application potential and is expected to be used in clinical screening and treatment detection of mycoplasma pneumoniae (MP) infection.

Graphical Abstract