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<p> A&#xa0;Cr-doped VO<sub>2</sub> nanobelt (Cr/VO<sub>2</sub>) with remarkable peroxidase-like activity was synthesized and coupled with uricase to catalyze the cascade reaction for&#xa0; detection of uric acid. Notably, the affinity of Cr/VO<sub>2</sub> for 3,3',5,5'-tetramethylbenzidine dihydrochloride hydrate (TMB) and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) is tenfold and 20-fold higher, respectively, than that of horseradish peroxidase (HRP). The Cr/VO<sub>2</sub> exhibits highly reactive and stable peroxidase activity at temperatures of 20-60 ℃. Thus, the reaction catalyzed by Cr/VO<sub>2</sub> can be carried out at room temperature, greatly simplifying the testing process. This enzyme cascade nanozyme based colorimetric assay enables the selective and simple detection of uric acid within 30 min with a detection limit of only 0.34&#xa0;μM, and has an observable potential in clinical applications.</p> Graphical abstract <p></p>

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Enzyme cascade nanozyme based colorimetric sensor for detection of uric acid as a biomarker of hyperuricemia

  • 期刊论文

  • 发表日期：

    2025年1月15日

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  • Wenrui Ke,
  • Guochen Kuang,
  • Chuangqi Gu,
  • Junhao Wang,
  • Xiao Jiang,
  • Rongjiao Zhu,
  • Haiyuan Wang,
  • Zhen Zhang

摘要

A Cr-doped VO2 nanobelt (Cr/VO2) with remarkable peroxidase-like activity was synthesized and coupled with uricase to catalyze the cascade reaction for  detection of uric acid. Notably, the affinity of Cr/VO2 for 3,3',5,5'-tetramethylbenzidine dihydrochloride hydrate (TMB) and hydrogen peroxide (H2O2) is tenfold and 20-fold higher, respectively, than that of horseradish peroxidase (HRP). The Cr/VO2 exhibits highly reactive and stable peroxidase activity at temperatures of 20-60 ℃. Thus, the reaction catalyzed by Cr/VO2 can be carried out at room temperature, greatly simplifying the testing process. This enzyme cascade nanozyme based colorimetric assay enables the selective and simple detection of uric acid within 30 min with a detection limit of only 0.34 μM, and has an observable potential in clinical applications.

Graphical abstract