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<p> A&#xa0;lateral flow assay (LFA) has been developed&#xa0;that can be used in point-of-care (PoC) use for the sensitive determination of leptin hormone. The limit of detection value was 0.158&#xa0;ng/mL and the limit of quantification value was 0.479&#xa0;ng/mL for leptin hormone. The stability studies showed that the assay response was equal to the initial value even after 6&#xa0;months. LFA accuracy tests performed in commercial artificial serum samples were compared with the enzyme-linked immunosorbent assay (ELISA). The obtained&#xa0;recovery values between 95 and 110% show that the developed LFA can be used for quantitative determination&#xa0;of leptin hormone. As the result of the study, a fast and disposable assay was developed for the determination of leptin hormone, which can be performed with a small amount of sample in less than 15&#xa0;min without the need for long-term analysis stages, qualified personnel, expensive equipment and devices.</p> Graphical Abstract <p></p>

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Quantitative determination of leptin hormone using gold nanoparticle-based lateral flow assay

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年1月9日

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  • Erhan Zor,
  • Sabri Alpaydin,
  • Haluk Bingol

摘要

A lateral flow assay (LFA) has been developed that can be used in point-of-care (PoC) use for the sensitive determination of leptin hormone. The limit of detection value was 0.158 ng/mL and the limit of quantification value was 0.479 ng/mL for leptin hormone. The stability studies showed that the assay response was equal to the initial value even after 6 months. LFA accuracy tests performed in commercial artificial serum samples were compared with the enzyme-linked immunosorbent assay (ELISA). The obtained recovery values between 95 and 110% show that the developed LFA can be used for quantitative determination of leptin hormone. As the result of the study, a fast and disposable assay was developed for the determination of leptin hormone, which can be performed with a small amount of sample in less than 15 min without the need for long-term analysis stages, qualified personnel, expensive equipment and devices.

Graphical Abstract