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<p>MiRNA-208a is a molecular marker for the early stage of acute myocardial infarction (AMI), with satisfactory specificity and a relatively short time window, making it highly suitable for the early diagnosis of AMI. In this study, a CuNCs-cDNA-MoS<sub>2</sub> fluorescent probe is designed&#xa0;and a Förster resonance energy transfer (FRET) enzyme-free fluorescent biosensor is established&#xa0;for “on-off-on” detection of miRNA-208a. The results showed that the detection limit for miRNA-208a was 900&#xa0;pM, with a linear range of 1 to 10&#xa0;nM. Interestingly, after the addition of RNase inhibitor, the detection time and limit for serum miRNA-208a were reduced to 5&#xa0;min and 380&#xa0;pM, respectively. It also has a wide linear range (1-20&#xa0;nM), with a recovery of 98.13 to 101.20%. Compared to traditional methods, this strategy is simple, rapid, efficient, and cost-effective. In summary, the FRET-based fluorescent sensor provides a high-performance method for detecting miRNA-208a.</p> Graphical Abstract <p></p>

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Luminescence “on-off-on” sensing of acute myocardial infarction biomarker miRNA-208a based on copper nanoclusters-MoS2 FRET system

  • 期刊论文

  • 发表日期：

    2025年2月15日

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  • Suzhen Zhang,
  • Danling Mei,
  • Hongming Fang,
  • Quanwei Li,
  • Xiumei Zhou,
  • Xueqin Zhao,
  • Zhencang Zheng,
  • Biao Huang

摘要

MiRNA-208a is a molecular marker for the early stage of acute myocardial infarction (AMI), with satisfactory specificity and a relatively short time window, making it highly suitable for the early diagnosis of AMI. In this study, a CuNCs-cDNA-MoS2 fluorescent probe is designed and a Förster resonance energy transfer (FRET) enzyme-free fluorescent biosensor is established for “on-off-on” detection of miRNA-208a. The results showed that the detection limit for miRNA-208a was 900 pM, with a linear range of 1 to 10 nM. Interestingly, after the addition of RNase inhibitor, the detection time and limit for serum miRNA-208a were reduced to 5 min and 380 pM, respectively. It also has a wide linear range (1-20 nM), with a recovery of 98.13 to 101.20%. Compared to traditional methods, this strategy is simple, rapid, efficient, and cost-effective. In summary, the FRET-based fluorescent sensor provides a high-performance method for detecting miRNA-208a.

Graphical Abstract